ArticleNature protocols2015
Using the chicken embryo to assess virulence of Listeria monocytogenes and to model other microbial infections.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Influenza A Virus (H1N1) Infection Induces Ferroptosis to Promote Developmental Injury in Fetal Tissues.Cell proliferation · 2026Pooled it
- Characterization ofFoods (Basel, Switzerland) · 2025Article
- Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria.Nature communications · 2024Article
- The type III secretion system facilitates systemic infections ofMicrobiology spectrum · 2024Article
- The Virulence and Infectivity of Listeria monocytogenes Are Not Substantially Altered by Elevated SigB Activity.Infection and immunity · 2023Article
- Stress Adaptation Responses of aFoods (Basel, Switzerland) · 2023Article
- Chicken embryo lethality assay for determining the lethal dose, tissue distribution and pathogenicity of clinical Enterococcus cecorum isolates from poultry.Scientific reports · 2022Article
- Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci.Scientific reports · 2021Article
- Pmp Repertoires Influence the Different Infectious Potential of Avian and MammalianFrontiers in microbiology · 2021Article
- The use of embryonic chicken eggs as an alternative model to evaluate the virulence of Salmonella enterica serovar Gallinarum.PloS one · 2020Article
- The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313.PLoS neglected tropical diseases · 2019Article
- Article
- Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model.Veterinary research · 2018Article
- Bovine originVeterinary world · 2017Article
- Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA.Cell chemical biology · 2016Article
- Avian embryo monitoring during incubation using multi-channel diffuse speckle contrast analysis.Biomedical optics express · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial infections are a global health problem, particularly as microbes are continually developing resistance to antimicrobial treatments. An effective and reliable method for testing the virulence of different microbial pathogens is therefore a useful research tool. This protocol describes how the chicken embryo can be used as a trustworthy, inexpensive, ethically desirable and quickly accessible model to assess the virulence of the human bacterial pathogen Listeria monocytogenes, which can also be extended to other microbial pathogens. We provide a step-by-step protocol and figures and videos detailing the method, including egg handling, infection strategies, pathogenicity screening and isolation of infected organs. From the start of incubation of the fertilized eggs, the protocol takes <4 weeks to complete, with the infection part taking only 3 d. We discuss the appropriate controls to use and potential adjustments needed for adapting the protocol for other microbial pathogens.
Indexed as
Identifiers
26134955What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.