ArticleNature protocols2015
An assay to probe Plasmodium falciparum growth, transmission stage formation and early gametocyte development.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Protocols for Plasmodium gametocyte production in vitro: an integrative review and analysis.Parasites & vectors · 2022Pooled it
- An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.Nature protocols · 2026Review
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Defining the signature of deformable and infectiousWellcome open research · 2026Article
- The inducible gametocyte producer (iGP1) strain is well-suited to produce both immature and mature gametocytes for subsequent drug sensitivity profiling.Frontiers in cellular and infection microbiology · 2026Article
- An all-in-one pipeline for the in vitro discovery and in vivo testing of Plasmodium falciparum malaria transmission blocking drugs.Nature communications · 2025Article
- Reversible host cell surface remodelling limits immune recognition and maximizes survival of Plasmodium falciparum gametocytes.PLoS pathogens · 2025Article
- Induction of gametocytogenesis in human malaria parasites: from stress to genome editing.Frontiers in microbiology · 2025Review
- Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens.Scientific reports · 2024Article
- Phenotypic Screens Identify Genetic Factors Associated with Gametocyte Development in the Human Malaria Parasite Plasmodium falciparum.Microbiology spectrum · 2023Article
- A novel class of sulphonamides potently block malaria transmission by targeting a Plasmodium vacuole membrane protein.Disease models & mechanisms · 2023Article
- High-throughput analysis of the transcriptional patterns of sexual genes in malaria.Parasites & vectors · 2023Article
- AnFrontiers in cellular and infection microbiology · 2023Article
- Revisiting the Effect of Pharmaceuticals on Transmission Stage Formation in the Malaria ParasiteFrontiers in cellular and infection microbiology · 2022Article
- CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research.Nature communications · 2021Article
- Genomic and Genetic Approaches to Studying Antimalarial Drug Resistance and Plasmodium Biology.Trends in parasitology · 2021Review
- Stressed Out AboutFrontiers in cellular and infection microbiology · 2021Review
- Lactic Acid Supplementation Increases Quantity and Quality of Gametocytes in Plasmodium falciparum Culture.Infection and immunity · 2020Article
- Metabolic regulation of sexual commitment in Plasmodium falciparum.Current opinion in microbiology · 2020Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Conversion from asexual proliferation to sexual differentiation initiates the production of the gametocyte, which is the malaria parasite stage required for human-to-mosquito transmission. This protocol describes an assay designed to probe the effect of drugs or other perturbations on asexual replication, sexual conversion and early gametocyte development in the major human malaria parasite Plasmodium falciparum. Synchronized asexually replicating parasites are induced for gametocyte production by the addition of conditioned medium, and they are then exposed to the treatment of interest during sexual commitment or at any subsequent stage of early gametocyte development. Flow cytometry is used to measure asexual proliferation and gametocyte production via DNA dye staining and the gametocyte-specific expression of a fluorescent protein, respectively. This screening approach may be used to identify and evaluate potential transmission-blocking compounds and to further investigate the mechanism of sexual conversion in malaria parasites. The full protocol can be completed in 11 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.