Evidence map›Paper›PMID 26134681›Full record

ArticleOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA2016

Alkaline biodegradable implants for osteoporotic bone defects--importance of microenvironment pH.

W Liu, T Wang, C Yang, B W Darvell, J Wu, K Lin, J Chang, H Pan, W W Lu

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Article in Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

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  19. Calcium Phosphate-Based Biomaterials for Bone Repair.Journal of functional biomaterials · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

W LiuDepartment of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
T WangShenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
C YangSchool of Medicine, Shenzhen University, Shenzhen, 518052, China.
B W DarvellDental Materials Science, Department of Bioclinical Sciences, Faculty of Dentistry, Kuwait University, Kuwait City, Kuwait.
J WuDepartment of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
K LinState Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
J ChangState Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, China.
H PanShenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. hb.pan@siat.ac.cn.
W W LuDepartment of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. wwlu@hku.hk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledChange of microenvironment pH by biodegradable implants may ameliorate unbalanced osteoporotic bone remodeling. The present work demonstrated that a weak alkaline condition stimulated osteoblasts differentiation while suppressed osteoclast generation. In vivo, implants with an alkaline microenvironment pH (monitored by a pH microelectrode) exhibited a promising healing effect for the repair of osteoporotic bone defects.

introductionUnder osteoporotic conditions, the response of the bone microenvironment to an endosseous implant is significantly impaired, and this substantially increases the risk of fracture, non-union and aseptic implant loosening. Acid-base equilibrium is an important factor influencing bone cell behaviour. The present purpose was to study the effect of a series of alkaline biodegradable implant materials on regeneration of osteoporotic bone defect, monitoring the microenvironment pH (μe-pH) over time.

methodsThe proliferation and differentiation potential of osteoporotic rat bone marrow stromal cells and RAW 264.7 cells were examined under various pH conditions. Ovariectomized rat bone defects were filled with specific biodegradable materials, and μe-pH was measured by pH microelectrode. New osteoid and tartrate-resistant acid phosphatase-positive osteoclast-like cells were examined by Goldner's trichrome and TRAP staining, respectively. The intermediate layer between implants and new bone were studied using energy-dispersive X-ray spectroscopy (EDX) linear scanning.

resultsIn vitro, weak alkaline conditions stimulated osteoporotic rat bone marrow stromal cells (oBMSC) differentiation, while inhibiting the formation of osteoclasts. In vivo, μe-pH differs from that of the homogeneous peripheral blood and exhibits variations over time particular to each material. Higher initial μe-pH was associated with more new bone formation, late response of TRAP-positive osteoclast-like cells and the development of an intermediate 'apatitic' layer in vivo. EDX suggested that residual material may influence μe-pH even 9 weeks post-surgery.

conclusionThe pH microelectrode is suitable for in vivo μe-pH detection. Alkaline biodegradable materials generate an in vivo microenvironmental pH which is higher than the normal physiological value and show promising healing effects in the context of osteoporotic bone defects.

Indexed as

Absorbable ImplantsOrthopedic Fixation DevicesAcid-Base EquilibriumAnimalsBiocompatible MaterialsBone RemodelingCell DifferentiationCell ProliferationCells, CulturedDisease Models, AnimalFemaleHydrogen-Ion ConcentrationImplants, ExperimentalMesenchymal Stem CellsMicroelectrodesOsteoblastsBiocompatible MaterialsBone remodelingIn vivo microenvironment pHOrthopaedic biomaterialsOsteoporotic bone defect

Identifiers

PMID26134681

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.