ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Spontaneous Vesicle Release Is Not Tightly Coupled to Voltage-Gated Calcium Channel-Mediated Ca2+ Influx and Is Triggered by a Ca2+ Sensor Other Than Synaptotagmin-2 at the Juvenile Mice Calyx of Held Synapses.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Functional Nano-segregation of Distinct Forms of Neurotransmission.Advances in neurobiology · 2026Review
- Synaptic Vesicle Recycling at the Developing Presynapse.Journal of neurochemistry · 2025Review
- Nano-organization of synaptic calcium signaling.Biochemical Society transactions · 2024Review
- Spatially non-overlapping CaCell reports · 2023Article
- Article
- Cannabinoid receptor activation acutely increases synaptic vesicle numbers by activating synapsins in human synapses.Molecular psychiatry · 2021Article
- Article
- Alternative Splicing of Presynaptic Neurexins Differentially Controls Postsynaptic NMDA and AMPA Receptor Responses.Neuron · 2019Article
- Developmental Aspects of Glucose and Calcium Availability on the Persistence of Memory Function Over the Lifespan.Frontiers in aging neuroscience · 2019Review
- Copine-6 Binds to SNAREs and Selectively Suppresses Spontaneous Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- SNT-1 Functions as the CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Calcium dependence of spontaneous neurotransmitter release.Journal of neuroscience research · 2018Review
- The First 100 nm Inside the Pre-synaptic Terminal Where Calcium Diffusion Triggers Vesicular Release.Frontiers in synaptic neuroscience · 2018Review
- Distinct Actions of Voltage-Activated CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Differences in Cognitive Function of Rats with Traumatic Brain Injuries Following Hyperbaric Oxygen Therapy.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well known that voltage-gated calcium channels (VGCCs)-mediated Ca(2+) influx triggers evoked synaptic vesicle release. However, the mechanisms of Ca(2+) regulation of spontaneous miniature vesicle release (mini) remain poorly understood. Here we show that blocking VGCCs at the juvenile mice (C57BL/6) calyx of Held synapse failed to cause an immediate change in minis. Instead, it resulted in a significant reduction (∼40%) of mini frequency several minutes after the blockage. By recording VGCC activity and single vesicle fusion events directly at the presynaptic terminal, we found that minis did not couple to VGCC-mediated Ca(2+) entry, arguing for a lack of direct correlation between mini and transient Ca(2+) influx. Moreover, mini frequencies displayed a lower apparent Ca(2+) cooperativity than those of evoked release. In agreement with this observation, abrogation of the Ca(2+) sensor synaptotagmin-2 had no effect on apparent Ca(2+) cooperativity of minis. Together, our study provides the first direct evidence that spontaneous minis are not mediated by transient Ca(2+) signals through VGCCs and are triggered by a Ca(2+)-sensing mechanism that is different from the evoked release at these microdomain VGCC-vesicle coupled synapses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.