Evidence map›Paper›PMID 26134564›Full record

ArticleThe Journal of biological chemistry2015

Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors.

Wei-Lun Hsu, Hui-Wen Chung, Chih-Yueh Wu, Huei-Ing Wu, Yu-Tao Lee, En-Chan Chen, Weilun Fang, Yen-Chung Chang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 39 citations in OpenAlex.

  1. Trial
  2. Review
  3. The Healthy and Diseased Retina Seen through Neuron-Glia Interactions.International journal of molecular sciences · 2024
    Review
  4. Review
  5. Article
  6. A Ribosomal Perspective on Neuronal Local Protein Synthesis.Frontiers in molecular neuroscience · 2022
    Review
  7. Article
  8. Article
  9. Downregulated AKT-mTOR signaling pathway proteins in dorsolateral prefrontal cortex in Schizophrenia.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2020
    Article
  10. mTOR at the nexus of nutrition, growth, ageing and disease.Nature reviews. Molecular cell biology · 2020
    Review
  11. Article
  12. Article
  13. Review
  14. Review
  15. Review
  16. Expanding Axonal Transcriptome Brings New Functions for Axonally Synthesized Proteins in Health and Disease.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2018
    Review
  17. Review
  18. AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Wei-Lun HsuInstitute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan.
Hui-Wen ChungInstitute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan.
Chih-Yueh WuInstitute of Systems Neuroscience, National Tsing Hua University, Hsinchu 300, Taiwan.
Huei-Ing WuInstitute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan.
Yu-Tao LeeDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
En-Chan ChenDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
Weilun FangDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
Yen-Chung ChangInstitute of Molecular Medicine, National Tsing Hua University, Hsinchu 300, Taiwan; Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu 300, Taiwan; Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan. Electronic address: ycchang@life.nthu.edu.tw.
National Tsing Hua University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glutamate is the principal excitatory neurotransmitter in the mammalian CNS. By analyzing the metabolic incorporation of azidohomoalanine, a methionine analogue, in newly synthesized proteins, we find that glutamate treatments up-regulate protein translation not only in intact rat cortical neurons in culture but also in the axons emitting from cortical neurons before making synapses with target cells. The process by which glutamate stimulates local translation in axons begins with the binding of glutamate to the ionotropic AMPA receptors and metabotropic glutamate receptor 1 and members of group 2 metabotropic glutamate receptors on the plasma membrane. Subsequently, the activated mammalian target of rapamycin (mTOR) signaling pathway and the rise in Ca(2+), resulting from Ca(2+) influxes through calcium-permeable AMPA receptors, voltage-gated Ca(2+) channels, and transient receptor potential canonical channels, in axons stimulate the local translation machinery. For comparison, the enhancement effects of brain-derived neurotrophic factor (BDNF) on the local protein synthesis in cortical axons were also studied. The results indicate that Ca(2+) influxes via transient receptor potential canonical channels and activated the mTOR pathway in axons also mediate BDNF stimulation to local protein synthesis. However, glutamate- and BDNF-induced enhancements of translation in axons exhibit different kinetics. Moreover, Ca(2+) and mTOR signaling appear to play roles carrying different weights, respectively, in transducing glutamate- and BDNF-induced enhancements of axonal translation. Thus, our results indicate that exposure to transient increases of glutamate and more lasting increases of BDNF would stimulate local protein synthesis in migrating axons en route to their targets in the developing brain.

Indexed as

Alaninealpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidAnimalsAxonsBrain-Derived Neurotrophic FactorCalcium ChannelsCalcium SignalingEmbryo, MammalianGene Expression Regulation, DevelopmentalGlutamic AcidHippocampusKineticsPrimary Cell CultureProtein BiosynthesisRatsRats, Sprague-DawleyAlaninealpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidazidohomoalanineBrain-Derived Neurotrophic FactorCalcium ChannelsGlutamic Acidmetabotropic glutamate receptor 2metabotropic glutamate receptor type 1mTOR protein, ratReceptors, AMPAReceptors, Metabotropic GlutamateTOR Serine-Threonine KinasesTRPV Cation Channelsaxonglutamatelocal translationmetabotropic glutamate receptor (mGluR)protein synthesisα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPA receptor, AMPAR)

Identifiers

PMID26134564
PMCPMC4543638
OpenAlexW1657674651

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.