Evidence map›Paper›PMID 26134408›Full record

ArticlePloS one2015

Cytotoxic Effect of a Novel Synthesized Carbazole Compound on A549 Lung Cancer Cell Line.

Refilwe P Molatlhegi, Alisa Phulukdaree, Krishnan Anand, Robert M Gengan, Charlette Tiloke, Anil A Chuturgoon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Refilwe P MolatlhegiDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, RSA.
Alisa PhulukdareeDepartment of Physiology, School of Medicine, University of Pretoria, Pretoria, RSA.
Krishnan AnandDepartment of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, RSA.
Robert M GenganDepartment of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, RSA.
Charlette TilokeDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, RSA.
Anil A ChuturgoonDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, RSA.
University of KwaZulu-Natal · ZADurban University of Technology · ZAUniversity of Pretoria · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increased death rates due to lung cancer have necessitated the search for potential novel anticancer compounds such as carbazole derivatives. Carbazoles are aromatic heterocyclic compounds with anticancer, antibacterial and anti-inflammatory activity. The study investigated the ability of the novel carbazole compound (Z)-4-[9-ethyl-9aH-carbazol-3-yl) amino] pent-3-en-2-one (ECAP) to induce cytotoxicity of lung cancer cells and its mechanism of action. ECAP was synthesized as a yellow powder with melting point of 240-247 °C. The 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lipid peroxidation and comet assays were used to assess the cytotoxic effect of the compound on A549 lung cancer cells. Protein expression was determined using western blots, apoptosis was measured by luminometry (caspase-3/7, -8 and -9) assay and flow cytometry was used to measure phosphatidylserine (PS) externalisation. ECAP induced a p53 mediated apoptosis of lung cancer cells due to a significant reduction in the expression of antioxidant defence proteins (Nrf2 and SOD), Hsp70 (p < 0.02) and Bcl-2 (p < 0.0006), thereby up-regulating reactive oxygen species (ROS) production. This resulted in DNA damage (p < 0.0001), up-regulation of Bax expression and caspase activity and induction of apoptosis in lung cancer cells. The results show the anticancer potential of ECAP on lung cancer.

Indexed as

Gene Expression Regulation, NeoplasticApoptosisbcl-2-Associated X ProteinCarbazolesCaspasesCell Line, TumorCell ProliferationCell SurvivalCytotoxinsEpithelial CellsHSP70 Heat-Shock ProteinsHumansLipid PeroxidationNF-E2-Related Factor 2Oxidative StressPentanones4-(9-ethyl-9aH-carbazol-3-yl)amino)pent-3-en-2-oneBAX protein, humanbcl-2-Associated X ProteinBCL2 protein, humanCarbazolesCaspasesCytotoxinsHSP70 Heat-Shock ProteinsNFE2L2 protein, humanNF-E2-Related Factor 2PentanonesPhosphatidylserinesProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesSuperoxide DismutaseTumor Suppressor Protein p53

Identifiers

PMID26134408
PMCPMC4489925
OpenAlexW839644325

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.