ArticleAnnals of botany2015
Global 5-methylcytosine alterations in DNA during ageing of Quercus robur seeds.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
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- Unravelling DNA methylation dynamics during developmental stages in Quercus ilex subsp. ballota [Desf.] Samp.BMC plant biology · 2024Article
- The methylation landscape of giga-genome and the epigenetic timer of age in Chinese pine.Nature communications · 2023Article
- Persistence of parental age effect on somatic mutation rates across generations in Arabidopsis.BMC plant biology · 2023Article
- Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage.International journal of molecular sciences · 2023Article
- Choosing the Right Path for the Successful Storage of Seeds.Plants (Basel, Switzerland) · 2022Review
- Triticale doubled haploid plant regeneration factors linked by structural equation modeling.Journal of applied genetics · 2022Article
- Genome-wide identification, phylogeny, and gene duplication of the epigenetic regulators in Fagaceae.Physiologia plantarum · 2022Article
- Deterioration in the Quality of RecalcitrantMetabolites · 2022Article
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- Use of High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) to Quantify Modified Nucleosides.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Can Forest Trees Cope with Climate Change?-Effects of DNA Methylation on Gene Expression and Adaptation to Environmental Change.International journal of molecular sciences · 2021Review
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- Main Challenges and Actions Needed to Improve Conservation and Sustainable Use of Our Crop Wild Relatives.Plants (Basel, Switzerland) · 2020Review
- Dissecting the Seed Maturation and Germination Processes in the Non-OrthodoxInternational journal of molecular sciences · 2020Article
- Reactive Oxygen Species (ROS) and Nucleic Acid Modifications During Seed Dormancy.Plants (Basel, Switzerland) · 2020Review
- Genetic and Epigenetic Stability in Rye Seeds under Different Storage Conditions: Ageing and Oxygen Effect.Plants (Basel, Switzerland) · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsEpigenetic regulation plays an important role in the management of plant growth, development and response to stress factors, and several reports have indicated that DNA methylation plays a critical role in seed development and viability. This study examines changes in 5-methylcytosine (m(5)C) levels in the DNA of seeds during ageing, a process that has important implications for plant conservation and agriculture.
methodsChanges in the global level of m(5)C were measured in mature seeds of oak, Quercus robur. The extent of DNA methylation was measured using a protocol based on two-dimensional thin-layer chromatography. Viability of seeds was determined by germination and seedling emergence tests. KEY
resultsAn ageing-related decrease in total m(5)C during storage of recalcitrant seeds was highly and significantly correlated with a decrease in seed viability, as reflected by a reduction in germination (r = 0·8880) and seedling emergence (r = 0·8269).
conclusionsThe decrease in viability during ageing of Q. robur seeds is highly correlated with a global decline in the amount of m(5)C in genomic DNA, and it is possible that this may represent a typical response to ageing and senescence in recalcitrant seeds. Potential mechanisms that drive changes in genomic DNA methylation during ageing are discussed, together with their implications for seed viability.
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