ArticleJournal of visualized experiments : JoVE2015
Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Article
- Dietary restriction shapes intergenerational ribosome abundance and early growth of Caenorhabditis elegans offspring.PLoS biology · 2026Article
- Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology.Lab on a chip · 2025Article
- The neuropeptide FLP-11 induces and self-inhibits sleep through the receptor DMSR-1 in Caenorhabiditis elegans.Current biology : CB · 2025Article
- A sleep-active neuron can promote survival while sleep behavior is disturbed.PLoS genetics · 2023Article
- C. elegans molting requires rhythmic accumulation of the Grainyhead/LSF transcription factor GRH-1.The EMBO journal · 2023Article
- Coupling of growth rate and developmental tempo reduces body size heterogeneity in C. elegans.Nature communications · 2022Article
- Interneuron control of C. elegans developmental decision-making.Current biology : CB · 2022Article
- Sleep Analysis in AdultThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Developmental function and state transitions of a gene expression oscillator in Caenorhabditis elegans.Molecular systems biology · 2020Article
- A wake-active locomotion circuit depolarizes a sleep-active neuron to switch on sleep.PLoS biology · 2020Article
- Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in AgingACS chemical biology · 2019Article
- Quantitative imaging of sleep behavior in Caenorhabditis elegans and larval Drosophila melanogaster.Nature protocols · 2019Article
- Sleep Counteracts Aging Phenotypes to Survive Starvation-Induced Developmental Arrest in C. elegans.Current biology : CB · 2018Article
- Automated detection and manipulation of sleep in C. elegans reveals depolarization of a sleep-active neuron during mechanical stimulation-induced sleep deprivation.Scientific reports · 2018Article
- On-chip functional neuroimaging with mechanical stimulation in Caenorhabditis elegans larvae for studying development and neural circuits.Lab on a chip · 2018Article
- A simple culture system for long-term imaging of individual C. elegans.Lab on a chip · 2017Article
- High-throughput screening in the C. elegans nervous system.Molecular and cellular neurosciences · 2017Review
- Analysis of the NK2 homeobox geneeLife · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Behavior is controlled by the nervous system. Calcium imaging is a straightforward method in the transparent nematode Caenorhabditis elegans to measure the activity of neurons during various behaviors. To correlate neural activity with behavior, the animal should not be immobilized but should be able to move. Many behavioral changes occur during long time scales and require recording over many hours of behavior. This also makes it necessary to culture the worms in the presence of food. How can worms be cultured and their neural activity imaged over long time scales? Agarose Microchamber Imaging (AMI) was previously developed to culture and observe small larvae and has now been adapted to study all life stages from early L1 until the adult stage of C. elegans. AMI can be performed on various life stages of C. elegans. Long-term calcium imaging is achieved without immobilizing the animals by using short externally triggered exposures combined with an electron multiplying charge-coupled device (EMCCD) camera recording. Zooming out or scanning can scale up this method to image up to 40 worms in parallel. Thus, a method is described to image behavior and neural activity over long time scales in all life stages of C. elegans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.