ArticleJournal of visualized experiments : JoVE2015
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.International journal of molecular sciences · 2025Review
- Optimization of the TeraTox Assay for Preclinical Teratogenicity Assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test.Chemical research in toxicology · 2022Article
- Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity.Current research in toxicology · 2022Article
- In vitro teratogenicity testing using a 3D, embryo-like gastruloid system.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods.Archives of toxicology · 2020Article
- Article
- Stem cell-based test methods.EXCLI journal · 2019Article
- Performance metrics ofEXCLI journal · 2019Article
- Pluripotent Stem Cells in Developmental Toxicity Testing: A Review of Methodological Advances.Toxicological sciences : an official journal of the Society of Toxicology · 2018Review
- Article
- Definition of transcriptome-based indices for quantitative characterization of chemically disturbed stem cell development: introduction of the STOP-ToxArchives of toxicology · 2017Article
- Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis.PloS one · 2017Article
- Comparison of a teratogenic transcriptome-based predictive test based on human embryonic versus inducible pluripotent stem cells.Stem cell research & therapy · 2016Article
- Highlight report: Cardiotoxicity screening.EXCLI journal · 2016Article
- A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors.Archives of toxicology · 2015Article
- Drug-induced mitochondrial impairment in liver cells.EXCLI journal · 2015Article
- Highlight report: New methods for quantification of bile canalicular dynamics.EXCLI journal · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient protocols to differentiate human pluripotent stem cells to various tissues in combination with -omics technologies opened up new horizons for in vitro toxicity testing of potential drugs. To provide a solid scientific basis for such assays, it will be important to gain quantitative information on the time course of development and on the underlying regulatory mechanisms by systems biology approaches. Two assays have therefore been tuned here for these requirements. In the UKK test system, human embryonic stem cells (hESC) (or other pluripotent cells) are left to spontaneously differentiate for 14 days in embryoid bodies, to allow generation of cells of all three germ layers. This system recapitulates key steps of early human embryonic development, and it can predict human-specific early embryonic toxicity/teratogenicity, if cells are exposed to chemicals during differentiation. The UKN1 test system is based on hESC differentiating to a population of neuroectodermal progenitor (NEP) cells for 6 days. This system recapitulates early neural development and predicts early developmental neurotoxicity and epigenetic changes triggered by chemicals. Both systems, in combination with transcriptome microarray studies, are suitable for identifying toxicity biomarkers. Moreover, they may be used in combination to generate input data for systems biology analysis. These test systems have advantages over the traditional toxicological studies requiring large amounts of animals. The test systems may contribute to a reduction of the costs for drug development and chemical safety evaluation. Their combination sheds light especially on compounds that may influence neurodevelopment specifically.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.