ArticleJournal of visualized experiments : JoVE2015
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Ultrasound-guided skeletal muscle biopsy technique permits measurement of structural, functional, cellular and biochemical properties.Scientific reports · 2026Article
- A primer on the methods of skeletal and cardiac muscle mechanics using permeabilized preparations.The Journal of general physiology · 2026Review
- Paraspinal muscle fibres demonstrate a complex relationship between contractile variability and function in canines with spinal degeneration.Scientific reports · 2025Article
- The effect of storing fiber, fiber bundle, and whole muscle in glycerinated solution on their passive elastic modulus.Scientific reports · 2025Article
- Residual force enhancement is not altered while force depression is amplified at the cellular level in old age.The Journal of experimental biology · 2025Article
- Strain-dependent dynamic re-alignment of collagen fibers in skeletal muscle extracellular matrix.Acta biomaterialia · 2024Article
- Thrombospondin-1 promotes fibro-adipogenic stromal expansion and contractile dysfunction of the diaphragm in obesity.JCI insight · 2024Article
- Collagen architecture and biomechanics of gracilis and adductor longus muscles from children with cerebral palsy.The Journal of physiology · 2024Article
- Insights into posttranslational regulation of skeletal muscle contractile function by the acetyltransferases, p300 and CBP.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Glycerol induced paraspinal muscle degeneration leads to hyper-kyphotic spinal deformity in wild-type mice.Scientific reports · 2023Article
- Modulating fast skeletal muscle contraction protects skeletal muscle in animal models of Duchenne muscular dystrophy.The Journal of clinical investigation · 2023Article
- Skeletal muscle aging and sarcopenia: Perspectives from mechanical studies of single permeabilized muscle fibers.Journal of biomechanics · 2023Review
- Eccentric contraction-induced strength loss in dystrophin-deficient muscle: Preparations, protocols, and mechanisms.The Journal of general physiology · 2023Article
- Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration.Acta biomaterialia · 2023Article
- Single skeletal muscle fiber mechanical properties: a muscle quality biomarker of human aging.European journal of applied physiology · 2022Review
- Effects of Sarcomere Activators and Inhibitors Targeting Myosin Cross-Bridges on CaMolecular pharmacology · 2022Article
- Investigating the active contractile function of the rat paraspinal muscles reveals unique cross-bridge kinetics in the multifidus.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2022Article
- Available In Vitro Models for Human Satellite Cells from Skeletal Muscle.International journal of molecular sciences · 2021Review
- Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans.Scientific reports · 2021Article
- A mutation in desmin makes skeletal muscle less vulnerable to acute muscle damage after eccentric loading in rats.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. Single muscle fiber studies are useful in both basic science and clinical studies. For basic studies, single muscle fiber contractility measurements allow investigation of fundamental mechanisms of force production, and analysis of muscle function in the context of genetic manipulations. Clinically, single muscle fiber studies provide useful insight into the impact of injury and disease on muscle function, and may be used to guide the understanding of muscular pathologies. In this video article we outline the steps required to prepare and isolate an individual skeletal muscle fiber segment, attach it to force-measuring apparatus, activate it to produce maximum isometric force, and estimate its cross-sectional area for the purpose of normalizing the force produced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.