ArticleAnnals of laboratory medicine2015
Isolation and Identification of Clostridium difficile Using ChromID C. difficile Medium Combined With Gram Staining and PRO Disc Testing: A Proposal for a Simple Culture Process.
Article in Annals of laboratory medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Optimized Surveillance Testing for ToxigenicmedRxiv : the preprint server for health sciences · 2026Article
- Antibiotic resistance patterns, toxin profiles, and toxin gene expression levels among Clostridioides difficile PCR ribotypes isolated from patients scheduled for colonoscopy.Molecular biology reports · 2025Article
- Prevalence of Non-ToxigenicAntibiotics (Basel, Switzerland) · 2023Article
- Massive intravascular hemolysis is an important factor in Clostridium perfringens-induced bacteremia.Internal and emergency medicine · 2022Article
- Comparative Evaluation of Three Immunoassays for the Simultaneous Detection ofMicroorganisms · 2022Article
- Massive Hydrothorax and Ascites as the Primary Manifestation of Infection WithFrontiers in pediatrics · 2020Article
- Simultaneous Detection ofAnnals of laboratory medicine · 2019Article
- Relevance of Autophagy Induction by Gastrointestinal Hormones: Focus on the Incretin-Based Drug Target and Glucagon.Frontiers in pharmacology · 2019Review
- Effect of the Synthetic Bile Salt Analog CamSA on the Hamster Model of Clostridium difficile Infection.Antimicrobial agents and chemotherapy · 2018Article
- A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.Clinical microbiology reviews · 2017Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChromID C. difficile agar (CDIF; bioMérieux, France), a chromogenic medium, allows for the isolation and identification of Clostridium difficile strains within 24 hr regardless of pretreatment of stool specimens with heat or alcohol shock. In the present study, we designed and evaluated a simple procedure for the implementation C. difficile cultures using CDIF medium in a tertiary hospital setting.
methodsWe designed a simple protocol for untreated stool specimens using CDIF medium followed by Gram staining and PRO disc (PRO disc K1532B, Key Scientific Products, USA) testing for the identification of C. difficile in colonies produced on CDIF agar. A total of 1,402 prospectively collected stool specimens from patients with suspected C. difficile infection were tested. The protocol was evaluated by phenotypic or molecular identification of C. difficile using Vitek 2 ANC card (bioMérieux) or 16S rDNA/tpi gene sequencing, respectively.
resultsOf 1,402 stool specimens, 650 isolates were cultured in CDIF. Overall, 235 (36.2%, 235/650) strains could be presumptively identified as C. difficile by using Gram staining and PRO disc testing. Of those, 231 (98.3%, 231/235) isolates were confirmed as true C. difficile by molecular assays.
conclusionsThe use of CDIF combined with Gram staining and PRO disc testing of untreated stool specimens would allow for isolation and accurate identification of C. difficile strains and would be advantageous in reducing the multistep process for C. difficile culture.
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