ArticleWorld journal of stem cells2015
Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction.
Article in World journal of stem cells, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mesenchymal Stem Cell Sheet Engineering: Refining Cell Delivery Strategies in Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2026Review
- MgO-enhanced β-TCP promotes osteogenesis in both in vitro and in vivo rat models.Scientific reports · 2024Article
- Application of vancomycin-impregnated calcium sulfate hemihydrate/nanohydroxyapatite/carboxymethyl chitosan injectable hydrogels combined with BMSC sheets for the treatment of infected bone defects in a rabbit model.BMC musculoskeletal disorders · 2022Article
- Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration.International journal of nanomedicine · 2022Review
- Silicate/zinc-substituted strontium apatite coating improves the osteoinductive properties of β-tricalcium phosphate bone graft substitute.BMC musculoskeletal disorders · 2021Article
- Effects of human urine-derived stem cells on the cementogenic differentiation of indirectly-cocultured periodontal ligament stem cells.American journal of translational research · 2020Article
- Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.International journal of oral science · 2019Review
- Article
- Osteogenesis of osteogenic matrix cell sheets preserved in culture medium in a rat model.Cell transplantation · 2018Article
- Implantation of Bone Marrow Stromal Cell Sheets Derived from Old Donors Supports Bone Tissue Formation.Tissue engineering and regenerative medicine · 2018Article
- Modifying oxygen tension affects bone marrow stromal cell osteogenesis for regenerative medicine.World journal of stem cells · 2017Article
- Calcium Concentration in Culture Medium as a Nondestructive and Rapid Marker of Osteogenesis.Cell transplantation · 2017Article
- Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets.Stem cell research & therapy · 2017Article
- Culturing bone marrow cells with dexamethasone and ascorbic acid improves osteogenic cell sheet structure.Bone & joint research · 2016Article
- The application of cell sheet engineering in the vascularization of tissue regeneration.Regenerative medicine · 2016Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate (TCP) constructs on bone formation in bone defects.
methodsOsteogenic matrix cell sheets were prepared from bone marrow stromal cells (BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds (1) seeded with BMSCs; (2) wrapped with osteogenic matrix cell sheets; or (3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk.
resultsIn bone defects, constructs implanted with cell sheets showed callus formation with segmental or continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone non-union. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness (mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP constructs.
conclusionThis technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects.
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