ArticleEuropean journal of nutrition2016
Effects of high doses of vitamin D3 on mucosa-associated gut microbiome vary between regions of the human gastrointestinal tract.
Article in European journal of nutrition, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
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Who cites it
136 citing papers in PubMed, 2 syntheses or guidelines pooled it, 250 citations in OpenAlex.
- The Association between Vitamin D and Gut Microbiota: A Systematic Review of Human Studies.Nutrients · 2021Pooled it
- Vitamin D and the gut microbiome: a systematic review of in vivo studies.European journal of nutrition · 2019Pooled it
- Randomized control trial of moderate dose vitamin D alters microbiota stability and metabolite networks in healthy adults.Microbiology spectrum · 2024Trial
- Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?The Journal of clinical endocrinology and metabolism · 2021Trial
- Trial
- The potential role of vitamin D supplementation as a gut microbiota modifier in healthy individuals.Scientific reports · 2020Trial
- Serum 25-hydroxyvitamin D concentration in childhood and risk of islet autoimmunity and type 1 diabetes: the TRIGR nested case-control ancillary study.Diabetologia · 2020Trial
- Effect of Vitamin D Supplementation on Faecal Microbiota: A Randomised Clinical Trial.Nutrients · 2019Trial
- Vitamin D and Phenylbutyrate Supplementation Does Not Modulate Gut Derived Immune Activation in HIV-1.Nutrients · 2019Trial
- Bolus Weekly Vitamin D3 Supplementation Impacts Gut and Airway Microbiota in Adults With Cystic Fibrosis: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial.The Journal of clinical endocrinology and metabolism · 2018Trial
- Trial
- Trial
- The vitamin D receptor story: discovery, control, and genomic reach.The Journal of endocrinology · 2026Review
- Vitamin D Levels and Uropathogen Distribution in Urinary Tract Infections: A Six-Year Retrospective Study from Cyprus.Medicina (Kaunas, Lithuania) · 2026Article
- Integrating Genetics and Environment to Find Causal Mechanisms for Multiple Sclerosis.European journal of immunology · 2026Review
- Effects of Helicobacter pylori infection on gastric mucosal microbiota.World journal of pediatrics : WJP · 2026Review
- The bile acid-gut microbiota-vitamin D axis: new insights into biliary atresia.Frontiers in cellular and infection microbiology · 2026Review
- Vitamin D in mucosal healing: therapeutic potential and current evidence in inflammatory bowel disease.Therapeutic advances in gastroenterology · 2026Review
- Skin-directed Solius UVB light therapy increases vitamin D levels and alters intestinal microbiome and metabolome homeostasis.Frontiers in microbiology · 2026Article
- Modelling the influence of vitamin D and probiotics on inflammation and the intestinal microbiota in older adults.Scientific reports · 2025Article
76 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeVitamin D is well known for its effects on bone mineralisation but has also been attributed immunomodulatory properties. It positively influences human health, but in vivo data describing vitamin D effects on the human gut microbiome are missing. We aimed to investigate the effects of oral vitamin D3 supplementation on the human mucosa-associated and stool microbiome as well as CD8(+) T cells in healthy volunteers.
methodsThis was an interventional, open-label, pilot study. Sixteen healthy volunteers (7 females, 9 males) were endoscopically examined to access a total of 7 sites. We sampled stomach, small bowel, colon, and stools before and after 8 weeks of vitamin D3 supplementation. Bacterial composition was assessed by pyrosequencing the 16S rRNA gene (V1-2), and CD8(+) T cell counts were determined by flow cytometry.
resultsVitamin D3 supplementation changed the gut microbiome in the upper GI tract (gastric corpus, antrum, and duodenum). We found a decreased relative abundance of Gammaproteobacteria including Pseudomonas spp. and Escherichia/Shigella spp. and increased bacterial richness. No major changes occurred in the terminal ileum, appendiceal orifice, ascending colon, and sigmoid colon or in stools, but the CD8(+) T cell fraction was significantly increased in the terminal ileum.
conclusionVitamin D3 modulates the gut microbiome of the upper GI tract which might explain its positive influence on gastrointestinal diseases, such as inflammatory bowel disease or bacterial infections. The local effects of vitamin D demonstrate pronounced regional differences in the response of the GI microbiome to external factors, which should be considered in future studies investigating the human microbiome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.