ArticleJournal of dental research2015
External Dentin Stimulation Induces ATP Release in Human Teeth.
Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Tooth Pulp Afferents and Transient Receptor Potential (TRP) Ion Channels as Key Regulators of Pulp Homeostasis, Inflammation, and Pain.International journal of molecular sciences · 2025Review
- Intracellular cAMP signaling-induced CaPflugers Archiv : European journal of physiology · 2025Article
- Increased Purinergic Signaling in Human Dental Pulps With Inflammatory Pain is Sex-Dependent.The journal of pain · 2024Article
- Review
- Immunohistochemistry as a detection tool for ion channels involved in dental pain signaling.The Saudi dental journal · 2022Review
- Pathogenesis, diagnosis and management of dentin hypersensitivity: an evidence-based overview for dental practitioners.BMC oral health · 2020Review
- Pannexin-1 Channel Regulates ATP Release in Epilepsy.Neurochemical research · 2020Review
- Ion Channels Involved in Tooth Pain.International journal of molecular sciences · 2019Review
- Purinergic Signaling Modulates Survival/Proliferation of Human Dental Pulp Stem Cells.Journal of dental research · 2019Article
- The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain.International journal of molecular sciences · 2019Review
- CD73 Controls Extracellular Adenosine Generation in the Trigeminal Nociceptive Nerves.Journal of dental research · 2017Article
- Ionotropic P2X ATP Receptor Channels Mediate Purinergic Signaling in Mouse Odontoblasts.Frontiers in physiology · 2017Article
- Purinergic Signalling: Therapeutic Developments.Frontiers in pharmacology · 2017Review
- Expression of Vesicular Nucleotide Transporter in Rat Odontoblasts.Acta histochemica et cytochemica · 2016Article
- Neuronal NTPDase3 Mediates Extracellular ATP Degradation in Trigeminal Nociceptive Pathway.PloS one · 2016Article
- Intercellular Odontoblast Communication via ATP Mediated by Pannexin-1 Channel and Phospholipase C-coupled Receptor Activation.Frontiers in physiology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
ATP is involved in neurosensory processing, including nociceptive transduction. Thus, ATP signaling may participate in dentin hypersensitivity and dental pain. In this study, we investigated whether pannexins, which can form mechanosensitive ATP-permeable channels, are present in human dental pulp. We also assessed the existence and functional activity of ecto-ATPase for extracellular ATP degradation. We further tested if ATP is released from dental pulp upon dentin mechanical or thermal stimulation that induces dentin hypersensitivity and dental pain and if pannexin or pannexin/gap junction channel blockers reduce stimulation-dependent ATP release. Using immunofluorescence staining, we demonstrated immunoreactivity of pannexin 1 and 2 in odontoblasts and their processes extending into the dentin tubules. Using enzymatic histochemistry staining, we also demonstrated functional ecto-ATPase activity within the odontoblast layer, subodontoblast layer, dental pulp nerve bundles, and blood vessels. Using an ATP bioluminescence assay, we found that mechanical or cold stimulation to the exposed dentin induced ATP release in an in vitro human tooth perfusion model. We further demonstrated that blocking pannexin/gap junction channels with probenecid or carbenoxolone significantly reduced external dentin stimulation-induced ATP release. Our results provide evidence for the existence of functional machinery required for ATP release and degradation in human dental pulp and that pannexin channels are involved in external dentin stimulation-induced ATP release. These findings support a plausible role for ATP signaling in dentin hypersensitivity and dental pain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.