ArticleScientific reports2015
Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- Trial
- Proteoform-specific enrichment of phosphopeptide isomers by polymer-based synthetic receptors.Nature chemical biology · 2026Article
- Advanced (Nano)material-Based Enrichment Strategies for Quantitative Phosphoproteomics Analysis by Mass Spectrometry.Advances in experimental medicine and biology · 2026Review
- Fundamentals, Synthetic Strategies and Applications of Non-Covalently Imprinted Polymers.Molecules (Basel, Switzerland) · 2024Review
- In situ digestion-assisted multi-template imprinted nanoparticles for efficient analysis of protein phosphorylation.Mikrochimica acta · 2023Article
- Article
- Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates.Analytical and bioanalytical chemistry · 2021Review
- The Use of Computational Methods for the Development of Molecularly Imprinted Polymers.Polymers · 2021Review
- Selective Enrichment of Histidine Phosphorylated Peptides Using Molecularly Imprinted Polymers.Analytical chemistry · 2021Article
- Synthesis and Evaluation of Non-Hydrolyzable Phospho-Lysine Peptide Mimics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Homozygous expression of the myofibrillar myopathy-associated p.W2710X filamin C variant reveals major pathomechanisms of sarcomeric lesion formation.Acta neuropathologica communications · 2020Article
- Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides.Mikrochimica acta · 2019Article
- Effect of shell structure of Ti-immobilized metal ion affinity chromatography core-shell magnetic particles for phosphopeptide enrichment.Scientific reports · 2019Article
- Let me infuse this for you - A way to solve the first YPIC challenge.EuPA open proteomics · 2019Article
- Phosphotyrosine profiling of human cerebrospinal fluid.Clinical proteomics · 2018Article
- Methionine residues around phosphorylation sites are preferentially oxidized in vivo under stress conditions.Scientific reports · 2017Article
- Extracellular Protein Phosphorylation, the Neglected Side of the Modification.Molecular & cellular proteomics : MCP · 2017Review
- Mutant desmin substantially perturbs mitochondrial morphology, function and maintenance in skeletal muscle tissue.Acta neuropathologica · 2016Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphospecific enrichment techniques and mass spectrometry (MS) are essential tools for comprehending the cellular phosphoproteome. Here, we report a fast and simple approach for low sequence-bias phosphoserine (pS) peptide capture and enrichment that is compatible with low biological or clinical sample input. The approach exploits molecularly imprinted polymers (MIPs, "plastic antibodies") featuring tight neutral binding sites for pS or pY that are capable of cross-reacting with phosphopeptides of protein proteolytic digests. The versatility of the resulting method was demonstrated with small samples of whole-cell lysate from human embryonic kidney (HEK) 293T cells, human neuroblastoma SH-SY5Y cells, mouse brain or human cerebrospinal fluid (CSF). Following pre-fractionation of trypsinized proteins by strong cation exchange (SCX) chromatography, pS-MIP enrichment led to the identification of 924 phosphopeptides in the HEK 293T whole-cell lysate, exceeding the number identified by TiO2-based enrichment (230). Moreover, the phosphopeptides were extracted with low sequence bias and showed no evidence for the characteristic preference of TiO2 for acidic amino acids (aspartic and glutamic acid). Applying the method to human CSF led to the discovery of 47 phosphopeptides belonging to 24 proteins and revealed three previously unknown phosphorylation sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.