ArticleAmerican journal of physiology. Endocrinology and metabolism2015
Characterization of lipid metabolism in a novel immortalized human hepatocyte cell line.
Article in American journal of physiology. Endocrinology and metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD.Journal of lipid research · 2026Article
- Modeling fatty liver disease and progression with stem cell derived hepatocytes.Scientific reports · 2026Article
- Dietary fat quantity and composition influence hepatic lipid metabolism and metabolic disease risk in humans.Disease models & mechanisms · 2025Review
- Liver organoids: updates on generation strategies and biomedical applications.Stem cell research & therapy · 2024Review
- Comparison of HepaRG and HepG2 cell lines to model mitochondrial respiratory adaptations in non‑alcoholic fatty liver disease.International journal of molecular medicine · 2024Article
- Immortalization Reversibility in the Context of Cell Therapy Biosafety.International journal of molecular sciences · 2023Review
- Differential Lipid Accumulation on HepG2 Cells Triggered by Palmitic and Linoleic Fatty Acids Exposure.Molecules (Basel, Switzerland) · 2023Article
- Customized liver organoids as an advancedInternational journal of biological sciences · 2023Review
- Article
- Review
- Fatty Acids and a High-Fat Diet Induce Epithelial-Mesenchymal Transition by Activating TGFβ and β-Catenin in Liver Cells.International journal of molecular sciences · 2021Article
- Anticholesterolemic Activity of Three Vegetal Extracts (Artichoke, Caigua, and Fenugreek) and Their Unique Blend.Frontiers in pharmacology · 2021Article
- In Vitro and In Vivo Models of Non-Alcoholic Fatty Liver Disease: A Critical Appraisal.Journal of clinical medicine · 2020Review
- Modifying nutritional substrates induces macrovesicular lipid droplet accumulation and metabolic alterations in a cellular model of hepatic steatosis.Physiological reports · 2020Article
- Human PNPLA3-I148M variant increases hepatic retention of polyunsaturated fatty acids.JCI insight · 2019Article
- Identification of the Secreted Proteins Originated from Primary Human Hepatocytes and HepG2 Cells.Nutrients · 2019Article
- A Human Liver-on-a-Chip Platform for Modeling Nonalcoholic Fatty Liver Disease.Advanced biosystems · 2019Article
- A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells.Experimental & molecular medicine · 2018Article
- The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus.Cell and tissue banking · 2017Article
- In vitro cellular models of human hepatic fatty acid metabolism: differences between Huh7 and HepG2 cell lines in human and fetal bovine culturing serum.Physiological reports · 2017Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
The development of hepatocyte cell models that represent fatty acid partitioning within the human liver would be beneficial for the study of the development and progression of nonalcoholic fatty liver disease (NAFLD). We sought to develop and characterize a novel human liver cell line (LIV0APOLY) to establish a model of lipid accumulation using a physiological mixture of fatty acids under low- and high-glucose conditions. LIV0APOLY cells were compared with a well-established cell line (HepG2) and, where possible, primary human hepatocytes. LIV0APOLY cells were found to proliferate and express some mature liver markers and were wild type for the PNPLA3 (rs738409) gene, whereas HepG2 cells carried the Ile(148)Met variant that is positively associated with liver fat content. Intracellular triglyceride content was higher in HepG2 than in LIV0APOLY cells; exposure to high glucose and/or exogenous fatty acids increased intracellular triglyceride in both cell lines. Triglyceride concentrations in media were higher from LIV0APOLY compared with HepG2 cells. Culturing LIV0APOLY cells in high glucose increased a marker of endoplasmic reticulum stress and attenuated insulin-stimulated Akt phosphorylation whereas low glucose and exogenous fatty acids increased AMPK phosphorylation. Although LIV0APOLY cells and primary hepatocytes stored similar amounts of exogenous fatty acids as triglyceride, more exogenous fatty acids were partitioned toward oxidation in the LIV0APOLY cells than in primary hepatocytes. LIV0APOLY cells offer the potential to be a renewable cellular model for studying the effects of exogenous metabolic substrates on fatty acid partitioning; however, their usefulness as a model of lipoprotein metabolism needs to be further explored.
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