ArticleAnalytical chemistry2015
Paper-Based RNA Extraction, in Situ Isothermal Amplification, and Lateral Flow Detection for Low-Cost, Rapid Diagnosis of Influenza A (H1N1) from Clinical Specimens.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluation of Lateral-Flow Assay for Rapid Detection of Influenza Virus.BioMed research international · 2020Pooled it
- User experience of home-based AbC-19 SARS-CoV-2 antibody rapid lateral flow immunoassay test.Scientific reports · 2022Trial
- Development of an isothermal point of care method for detection of human papilloma virus (HPV).Aspects of molecular medicine · 2026Article
- Electrochemical Lateral Flow Platforms: Pioneering the Future of Rapid Testing.Molecules (Basel, Switzerland) · 2026Review
- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Paper-Based Microfluidic Chips for At-Home Point-of-Care Nucleic Acid Testing: Applications and Challenges.Diagnostics (Basel, Switzerland) · 2026Review
- Validation and application of a visual LAMP assay for Mpox diagnosis - Insights from clinical samples in Thailand.MethodsX · 2025Article
- Extraction-free RT-RPA assay for detection of HPV16, HPV18, and HPV45 mRNA expression.Scientific reports · 2025Article
- A novel method for semi-quantitative detection of HPV16 and HPV18 mRNA with a low-cost, open-source fluorimeter.Analytical and bioanalytical chemistry · 2025Article
- Article
- Influenza viruses and SARS-CoV-2 diagnosis via sensitive testing methods in clinical application.Heliyon · 2024Review
- Large-volume RT-LAMP enables extraction-free amplification of HIV RNA from fingerstick plasma.Analytica chimica acta · 2024Article
- PCR Independent Strategy-Based Biosensors for RNA Detection.Biosensors · 2024Review
- The peculiar characteristics and advancement in diagnostic methodologies of influenza A virus.Frontiers in microbiology · 2024Review
- Point-of-care testing for COVID-19: a simple two-step molecular diagnostic development and validation during the SARS-CoV-2 pandemic.Memorias do Instituto Oswaldo Cruz · 2024Article
- Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices.Analytical and bioanalytical chemistry · 2023Article
- CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The 2009 Influenza A (H1N1) pandemic disproportionately affected the developing world and highlighted the key inadequacies of traditional diagnostic methods that make them unsuitable for use in resource-limited settings, from expensive equipment and infrastructure requirements to unacceptably long turnaround times. While rapid immunoassay diagnostic tests were much less costly and more context-appropriate, they suffered from drastically low sensitivities and high false negative rates. An accurate, sensitive, and specific molecular diagnostic that is also rapid, low-cost, and independent of laboratory infrastructure is needed for effective point-of-care detection and epidemiological control in these developing regions. We developed a paper-based assay that allows for the extraction and purification of RNA directly from human clinical nasopharyngeal specimens through a poly(ether sulfone) paper matrix, H1N1-specific in situ isothermal amplification directly within the same paper matrix, and immediate visual detection on lateral flow strips. The complete sample-to-answer assay can be performed at the point-of-care in just 45 min, without the need for expensive equipment or laboratory infrastructure, and it has a clinically relevant viral load detection limit of 10(6) copies/mL, offering a 10-fold improvement over current rapid immunoassays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.