ArticleBioMed research international2015
Quantification of Internalized Silica Nanoparticles via STED Microscopy.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Recent Advances in Fluorescent Nanoparticles for Stimulated Emission Depletion Imaging.Biosensors · 2024Review
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Digital research data: from analysis of existing standards to a scientific foundation for a modular metadata schema in nanosafety.Particle and fibre toxicology · 2022Review
- Surface Treatment With Hydrophobic Coating Reagents (Organosilanes) Strongly Reduces the Bioactivity of Synthetic Amorphous SilicaFrontiers in public health · 2022Article
- Kinetics of nanoparticle uptake into and distribution in human cells.Nanoscale advances · 2021Review
- Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner.Nanomaterials (Basel, Switzerland) · 2021Article
- Nanoscopy for endosomal escape quantification.Nanoscale advances · 2021Review
- Shedding New Lights Into STED Microscopy: Emerging Nanoprobes for Imaging.Frontiers in chemistry · 2021Review
- Detection of large extracellular silver nanoparticle rings observed during mitosis using darkfield microscopy.PloS one · 2020Article
- Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix.RSC advances · 2019Article
- Biophysical comparison of four silver nanoparticles coatings using microscopy, hyperspectral imaging and flow cytometry.PloS one · 2019Article
- Distribution of SiOInternational journal of nanomedicine · 2019Article
- In Vitro and In Vivo Short-Term Pulmonary Toxicity of Differently Sized Colloidal Amorphous SiO₂.Nanomaterials (Basel, Switzerland) · 2018Article
- ISD3: a particokinetic model for predicting the combined effects of particle sedimentation, diffusion and dissolution on cellular dosimetry for in vitro systems.Particle and fibre toxicology · 2018Article
- Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy.Beilstein journal of nanotechnology · 2017Article
- Semi-automated quantification of living cells with internalized nanostructures.Journal of nanobiotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of safe engineered nanoparticles (NPs) requires a detailed understanding of their interaction mechanisms on a cellular level. Therefore, quantification of NP internalization is crucial to predict the potential impact of intracellular NP doses, providing essential information for risk assessment as well as for drug delivery applications. In this study, the internalization of 25 nm and 85 nm silica nanoparticles (SNPs) in alveolar type II cells (A549) was quantified by application of super-resolution STED (stimulated emission depletion) microscopy. Cells were exposed to equal particle number concentrations (9.2 × 10(10) particles mL(-1)) of each particle size and the sedimentation of particles during exposure was taken into account. Microscopy images revealed that particles of both sizes entered the cells after 5 h incubation in serum supplemented and serum-free medium. According to the in vitro sedimentation, diffusion, and dosimetry (ISDD) model 20-27% of the particles sedimented. In comparison, 10(2)-10(3) NPs per cell were detected intracellularly serum-containing medium. Furthermore, in the presence of serum, no cytotoxicity was induced by the SNPs. In serum-free medium, large agglomerates of both particle sizes covered the cells whereas only high concentrations (≥ 3.8 × 10(12) particles mL(-1)) of the smaller particles induced cytotoxicity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.