ArticleThe EMBO journal2015
Membrane protrusion powers clathrin-independent endocytosis of interleukin-2 receptor.
Article in The EMBO journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- From molecular mechanisms of glycosphingolipid function to bio-therapeutic applications.Cellular and molecular life sciences : CMLS · 2026Review
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Podocyte Endocytosis in Regulating the Glomerular Filtration Barrier.Frontiers in medicine · 2022Review
- CLN3, at the crossroads of endocytic trafficking.Neuroscience letters · 2021Article
- Cytokine receptor cluster size impacts its endocytosis and signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanisms and functions of endocytosis in T cells.Cell communication and signaling : CCS · 2021Review
- Endocytosis in the context-dependent regulation of individual and collective cell properties.Nature reviews. Molecular cell biology · 2021Review
- Multiple roles for actin in secretory and endocytic pathways.Current biology : CB · 2021Review
- Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.eLife · 2021Article
- New insights into the molecular mechanisms of axon guidance receptor regulation and signaling.Current topics in developmental biology · 2021Review
- HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease.Science (New York, N.Y.) · 2020Article
- Molecular mechanism of Fast Endophilin-Mediated Endocytosis.The Biochemical journal · 2020Review
- Article
- Correlative SICM-FCM reveals changes in morphology and kinetics of endocytic pits induced by disease-associated mutations in dynamin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Actomyosin-driven force patterning controls endocytosis at the immune synapse.Nature communications · 2019Article
- Inception Mechanisms of Tunneling Nanotubes.Cells · 2019Review
- The best of both worlds-bringing together cell biology and infection at the Institut Pasteur.Genes and immunity · 2019Review
- DOC-LS, a new liposome for dermal delivery, and its endocytosis by HaCaT and CCC-ESF-1 cells.IET nanobiotechnology · 2018Article
- Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function.Scientific reports · 2018Article
- Guided by curvature: shaping cells by coupling curved membrane proteins and cytoskeletal forces.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endocytosis controls many functions including nutrient uptake, cell division, migration and signal transduction. A clathrin- and caveolin-independent endocytosis pathway is used by important physiological cargos, including interleukin-2 receptors (IL-2R). However, this process lacks morphological and dynamic data. Our electron microscopy (EM) and tomography studies reveal that IL-2R-pits and vesicles are initiated at the base of protrusions. We identify the WAVE complex as a specific endocytic actor. The WAVE complex interacts with IL-2R, via a WAVE-interacting receptor sequence (WIRS) present in the receptor polypeptide, and allows for receptor clustering close to membrane protrusions. In addition, using total internal reflection fluorescent microscopy (TIRF) and automated analysis we demonstrate that two timely distinct bursts of actin polymerization are required during IL-2R uptake, promoted first by the WAVE complex and then by N-WASP. Finally, our data reveal that dynamin acts as a transition controller for the recruitment of Arp2/3 activators required for IL-2R endocytosis. Altogether, our work identifies the spatio-temporal specific role of factors initiating clathrin-independent endocytosis by a unique mechanism that does not depend on the deformation of a flat membrane, but rather on that of membrane protrusions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.