Evidence map›Paper›PMID 26124292›Full record

ArticleThe Journal of cell biology2015

Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes.

Melpomeni Platani, Laura Trinkle-Mulcahy, Michael Porter, A Arockia Jeyaprakash, William C Earnshaw

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Role of Diet in Stem and Cancer Stem Cells.International journal of molecular sciences · 2022
    Review
  5. GATOR2 complex-mediated amino acid signaling regulates brain myelination.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Melpomeni PlataniWellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK m.platani@ed.ac.uk Bill.Earnshaw@ed.ac.uk.
Laura Trinkle-MulcahyDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario K1H8M5, Canada Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario K1H8M5, Canada.
Michael PorterCentre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK.
A Arockia JeyaprakashWellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
William C EarnshawWellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK m.platani@ed.ac.uk Bill.Earnshaw@ed.ac.uk.
University of Edinburgh · GBUniversity of Dundee · GBUniversity of Ottawa · CAWellcome Centre for Cell Biology · GB

Funding

Wellcome Trust 073915Wellcome Trust 086194/Z/08/ZWellcome Trust 095822Wellcome Trust 095931Wellcome Trust 095931/Z/11/Z
6 · The paper itself

Abstract

Coordination of cell growth and proliferation in response to nutrient supply is mediated by mammalian target of rapamycin (mTOR) signaling. In this study, we report that Mio, a highly conserved member of the SEACAT/GATOR2 complex necessary for the activation of mTORC1 kinase, plays a critical role in mitotic spindle formation and subsequent chromosome segregation by regulating the proper concentration of active key mitotic kinases Plk1 and Aurora A at centrosomes and spindle poles. Mio-depleted cells showed reduced activation of Plk1 and Aurora A kinase at spindle poles and an impaired localization of MCAK and HURP, two key regulators of mitotic spindle formation and known substrates of Aurora A kinase, resulting in spindle assembly and cytokinesis defects. Our results indicate that a major function of Mio in mitosis is to regulate the activation/deactivation of Plk1 and Aurora A, possibly by linking them to mTOR signaling in a pathway to promote faithful mitotic progression.

Indexed as

Amino Acid SequenceAurora Kinase ACell Cycle ProteinsCentrosomeEnzyme ActivationGene Knockdown TechniquesHeLa CellsHumansKinesinsMitosisMolecular Sequence DataNeoplasm ProteinsNuclear Pore Complex ProteinsPolo-Like Kinase 1Protein Serine-Threonine KinasesProtein Structure, TertiaryAURKA protein, humanAurora Kinase ACell Cycle ProteinsDLGAP5 protein, humanKIF2C protein, humanKinesinsMTOR protein, humanNeoplasm ProteinsNuclear Pore Complex ProteinsNUP107 protein, humanPolo-Like Kinase 1Protein Serine-Threonine KinasesProto-Oncogene ProteinsTOR Serine-Threonine Kinases

Identifiers

PMID26124292
PMCPMC4494011
OpenAlexW1535915839

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.