Evidence map›Paper›PMID 26124283›Full record

ArticleMolecular and cellular biology2015

MicroRNA 665 Regulates Dentinogenesis through MicroRNA-Mediated Silencing and Epigenetic Mechanisms.

Hannah M Heair, Austin G Kemper, Bhaskar Roy, Helena B Lopes, Harunur Rashid, John C Clarke, Lubana K Afreen, Emanuela P Ferraz, Eddy Kim, Amjad Javed and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. TiInternational journal of nanomedicine · 2025
    Article
  3. METTL3-mediated pre-miR-665/DLX3 mExperimental & molecular medicine · 2024
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. MICROmanagement of Runx2 Function in Skeletal Cells.Current molecular biology reports · 2019
    Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Hannah M HeairDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Austin G KemperDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Bhaskar RoyDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Helena B LopesCell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Harunur RashidDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
John C ClarkeDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Lubana K AfreenDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Emanuela P FerrazCell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Eddy KimDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Amjad JavedDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Marcio M BelotiCell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Mary MacDougallDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Mohammad Q HassanDepartment of Oral and Maxillofacial Surgery, Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA hassank@uab.edu.
University of Alabama at Birmingham · USUniversidade de São Paulo · BR

Funding

Sp7 Mediated Control of Runx2 Function for Osteoblast DifferentiationR01AR062091 · NIAMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI JAVED, AMJAD · 2012 to 2025
$3.4M
Runx2 Controlled Adipocytic Differentiation in Aging MusculoskeletonR01AG030228 · NIA · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI JAVED, AMJAD · 2006 to 2009
$952k
NIAMS NIH HHS R01 AR062091NIA NIH HHS R01 AG030228
6 · The paper itself

Abstract

Studies of proteins involved in microRNA (miRNA) processing, maturation, and silencing have indicated the importance of miRNAs in skeletogenesis, but the specific miRNAs involved in this process are incompletely defined. Here, we identified miRNA 665 (miR-665) as a potential repressor of odontoblast maturation. Studies with cultured cell lines and primary embryonic cells showed that miR-665 represses the expression of early and late odontoblast marker genes and stage-specific proteases involved in dentin maturation. Notably, miR-665 directly targeted Dlx3 mRNA and decreased Dlx3 expression. Furthermore, RNA-induced silencing complex (RISC) immunoprecipitation and biotin-labeled miR-665 pulldown studies identified Kat6a as another potential target of miR-665. KAT6A interacted physically and functionally with RUNX2, activating tissue-specific promoter activity and prompting odontoblast differentiation. Overexpression of miR-665 reduced the recruitment of KAT6A to Dspp and Dmp1 promoters and prevented KAT6A-induced chromatin remodeling, repressing gene transcription. Taken together, our results provide novel molecular evidence that miR-665 functions in an miRNA-epigenetic regulatory network to control dentinogenesis.

Indexed as

AnimalsCell DifferentiationCell LineChromatinCore Binding Factor Alpha 1 SubunitDentinDentinogenesisDentin SialophosphoproteinExtracellular Matrix ProteinsGene Expression Regulation, DevelopmentalGene SilencingHEK293 CellsHistone AcetyltransferasesHomeodomain ProteinsHumansMiceChromatinCore Binding Factor Alpha 1 SubunitDentin SialophosphoproteinDistal-less homeobox proteinsDMP1 protein, humanExtracellular Matrix ProteinsHistone AcetyltransferasesHomeodomain ProteinsKAT6A protein, humanMicroRNAsMIRN665 microRNA, humanPeptide HydrolasesPhosphoproteinsRUNX2 protein, humanSialoglycoproteinsTranscription Factors

Identifiers

PMID26124283
PMCPMC4539367
OpenAlexW2216105743

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.