ArticleThe American journal of tropical medicine and hygiene2015
Detection of Entamoeba histolytica by Recombinase Polymerase Amplification.
Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Evaluation of Each ThreeMicroorganisms · 2025Article
- Rapid Detection of Amoebic Liver Abscess Using Recombinase Polymerase Amplification Assay in Resource-Limited Settings.The American journal of tropical medicine and hygiene · 2025Article
- Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection ofFrontiers in cellular and infection microbiology · 2024Article
- Development and application of recombinase polymerase amplification assay for rapid detection ofParasitology · 2023Article
- Prevalence of Human IntestinalPathogens (Basel, Switzerland) · 2022Review
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
- Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers.Children (Basel, Switzerland) · 2021Review
- Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19.Journal of virological methods · 2021Article
- Foodborne Parasitic Diseases in the Neotropics - A Review.Helminthologia · 2021Article
- Developments in integrating nucleic acid isothermal amplification and detection systems for point-of-care diagnostics.Biosensors & bioelectronics · 2020Review
- Direct detection of methicillin-resistant in Staphylococcus spp. in positive blood culture by isothermal recombinase polymerase amplification combined with lateral flow dipstick assay.World journal of microbiology & biotechnology · 2020Article
- DNA-Detection Based Diagnostics forJournal of parasitology research · 2020Review
- Influence of design probe and sequence mismatches on the efficiency of fluorescent RPA.World journal of microbiology & biotechnology · 2019Article
- Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay.PLoS neglected tropical diseases · 2019Article
- Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.Scientific reports · 2018Article
- Detection of Babesia gibsoni in dogs by combining recombinase polymerase amplification (RPA) with lateral flow (LF) dipstick.Parasitology research · 2018Article
- Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification.Acta tropica · 2018Article
- Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay.Scientific reports · 2016Article
- A Novel Isothermal Assay of Borrelia burgdorferi by Recombinase Polymerase Amplification with Lateral Flow Detection.International journal of molecular sciences · 2016Article
- Molecular Epidemiology of Entamoeba: First Description of Entamoeba moshkovskii in a Rural Area from Central Colombia.PloS one · 2015Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.