ArticleThe American journal of tropical medicine and hygiene2015
Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei.
Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- Development and international multicenter evaluation of a second-generation immunochromatography test for the serological diagnosis of melioidosis.PLoS neglected tropical diseases · 2026Article
- Evaluation of culture- and PCR-based methods for detecting Burkholderia pseudomallei in soil samples in Thailand.PLoS neglected tropical diseases · 2026Article
- An outer membrane vesicle vaccine prevents lung pathology in a macaque model of pneumonic melioidosis.Nature communications · 2025Article
- Evidence of populational Burkholderia pseudomallei exposure in Madagascar.PLoS neglected tropical diseases · 2025Article
- Occupational Laboratory Exposures toPathogens (Basel, Switzerland) · 2025Article
- Melioidosis of the Parotid Gland: Lessons Learned from a Fatal Case.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2025Article
- Performance of Antibody-Detection Tests for Human Melioidosis: A Systematic Review and Meta-analysis.The Malaysian journal of medical sciences : MJMS · 2024Review
- Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison.BMC veterinary research · 2024Article
- Development of a Novel Internally Controlled HrpB1 Gene-Based Real-Time qPCR Assay for Detection of Burkholderia pseudomallei.Molecular diagnosis & therapy · 2024Article
- Evaluating anti-GPL-core IgA as a diagnostic tool for non-tuberculous mycobacterial infections in Thai patients with high antibody background.Scientific reports · 2023Article
- Prospective Analysis of Antibody Diagnostic Tests and TTS1 Real-Time PCR for Diagnosis of Melioidosis in Areas Where It Is Endemic.Journal of clinical microbiology · 2023Article
- Development and evaluation of a multiplex serodiagnostic bead assay (BurkPx) for accurate melioidosis diagnosis.PLoS neglected tropical diseases · 2023Article
- Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a.PLoS neglected tropical diseases · 2022Article
- Evaluation of antigen-detecting and antibody-detecting diagnostic test combinations for diagnosing melioidosis.PLoS neglected tropical diseases · 2021Article
- Humoral Immune Responses toVeterinary sciences · 2020Article
- Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis.Journal of clinical microbiology · 2020Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Human Immune Responses to Melioidosis and Cross-Reactivity to Low-Virulence Burkholderia Species, ThailandEmerging infectious diseases · 2020Observational
- Distinct classes and subclasses of antibodies to hemolysin co-regulated protein 1 and O-polysaccharide and correlation with clinical characteristics of melioidosis patients.Scientific reports · 2019Article
- Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or non-endemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.