ArticleJournal of neural transmission (Vienna, Austria : 1996)2015
Enrichment of single neurons and defined brain regions from human brain tissue samples for subsequent proteome analysis.
Article in Journal of neural transmission (Vienna, Austria : 1996), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- The Aging Substantia Nigra is Characterized by ROS Accumulation Potentially Resulting in Increased Neuroinflammation and Cytoskeletal Remodeling.Advanced biology · 2025Article
- Don't die like me: Which proteins are responsible for the selective neuronal vulnerability within the substantia nigra?PloS one · 2024Article
- Neuronal subclass-selective proteomic analysis in Caenorhabditis elegans.Scientific reports · 2020Article
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- IgA autoantibodies against native myelin basic protein in a patient with MS.Neurology(R) neuroimmunology & neuroinflammation · 2019Article
- The use of localized proteomics to identify the drivers of Alzheimer's disease pathogenesis.Neural regeneration research · 2017Article
- Article
- Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF.Frontiers in molecular neuroscience · 2017Article
- Proteomic characterization of neuromelanin granules isolated from human substantia nigra by laser-microdissection.Scientific reports · 2016Article
- Proteomic analysis of neurons microdissected from formalin-fixed, paraffin-embedded Alzheimer's disease brain tissue.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 5 countries.
Funding
Abstract
Brain function in normal aging and neurological diseases has long been a subject of interest. With current technology, it is possible to go beyond descriptive analyses to characterize brain cell populations at the molecular level. However, the brain comprises over 100 billion highly specialized cells, and it is a challenge to discriminate different cell groups for analyses. Isolating intact neurons is not feasible with traditional methods, such as tissue homogenization techniques. The advent of laser microdissection techniques promises to overcome previous limitations in the isolation of specific cells. Here, we provide a detailed protocol for isolating and analyzing neurons from postmortem human brain tissue samples. We describe a workflow for successfully freezing, sectioning and staining tissue for laser microdissection. This protocol was validated by mass spectrometric analysis. Isolated neurons can also be employed for western blotting or PCR. This protocol will enable further examinations of brain cell-specific molecular pathways and aid in elucidating distinct brain functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.