ArticleBMC biotechnology2015
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Decreases in polyunsaturated fatty acid content improve heat stress tolerance during flowering and silicle development in pennycress (Frontiers in plant science · 2026Article
- Insights into the special physiology of Mortierella alpina cultured by agar supported solid state fermentation in enhancing arachidonic acid enriched lipid production.Scientific reports · 2025Article
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Design of high-monounsaturated fatty acid soybean seed oil using GmPDCTs knockout via a CRISPR-Cas9 system.Plant biotechnology journal · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- The core triacylglycerol toolbox in woody oil plants reveals targets for oil production bioengineering.Frontiers in plant science · 2023Article
- Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in YeastJournal of fungi (Basel, Switzerland) · 2022Review
- High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage.Biotechnology for biofuels · 2021Article
- Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT.Plant physiology · 2020Article
- A transferase interactome that may facilitate channeling of polyunsaturated fatty acid moieties from phosphatidylcholine to triacylglycerol.The Journal of biological chemistry · 2019Article
- Expression of a LycheePlant physiology · 2019Article
- Gene expression profiles that shape high and low oil content sesames.BMC genetics · 2019Article
- PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environments.Biotechnology for biofuels · 2019Article
- Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes.Plant cell reports · 2018Article
- De novo transcriptome assembly of the eight major organs of Sacha Inchi (Plukenetia volubilis) and the identification of genes involved in α-linolenic acid metabolism.BMC genomics · 2018Article
- Fatty Acid and Associated Gene Expression Analyses of Three Tree Peony Species Reveal Key Genes for α-Linolenic Acid Synthesis in Seeds.Frontiers in plant science · 2018Article
- Carbon and Acyl Chain Flux during Stress-induced Triglyceride Accumulation by Stable Isotopic Labeling of the Polar Microalga Coccomyxa subellipsoidea C169.The Journal of biological chemistry · 2017Article
- Expression ofFrontiers in plant science · 2017Article
- Acyl-Trafficking During Plant Oil Accumulation.Lipids · 2015Article
- In Vivo and in Vitro Evidence for Biochemical Coupling of Reactions Catalyzed by Lysophosphatidylcholine Acyltransferase and Diacylglycerol Acyltransferase.The Journal of biological chemistry · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
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Abstract
backgroundFlax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 (cisΔ9, 12, 15); ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications. The recently identified enzyme: phosphatidylcholine diacylglycerol cholinephosphotransferase (PDCT), catalyzes the interconversion between phosphatidylcholine (PC) and diacylglycerol (DAG), and has been shown to play an important role in PUFA accumulation in Arabidopsis thaliana seeds.
methodsTwo flax PDCT genes were identified using homology-based approach.
resultsIn this study, we describe the isolation and characterization of two PDCT genes from flax (LuPDCT1 and LuPDCT2) with very high nucleotide sequence identity (97%) whose deduced amino acid sequences exhibited approximately 55% identity with that of A. thaliana PDCT (AtROD1). The genes encoded functionally active enzymes that were strongly expressed in developing embryos. Complementation studies with the A. thaliana rod1 mutant demonstrated that the flax PDCTs were capable of restoring PUFA levels in planta. Furthermore, PUFA levels increased in Saccharomyces cerevisiae when the flax PDCTs were co-expressed with FATTY ACID DESATURASES (FADs), FAD2 and FAD3, while seed-specific expression of LuPDCT1 and LuPDCT2 in A. thaliana resulted in 16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid (18:1 (cisΔ9); OA).
conclusionsThe two novel PDCT homologs from flax are capable of increasing C18-PUFA levels substantially in metabolically engineered yeast and transgenic A. thaliana seeds. These flax PDCT proteins appear to play an important dual role in the determination of PUFA content by efficiently channelling monounsaturated FAs into PC for desaturation and moving the resulting PUFAs out of PC for subsequent use in TAG synthesis. These results indicate that flax PDCTs would be useful for bioengineering of oil crops to increase PUFA levels for applications in human food and nutritional supplements, animal feed and industrial bioproducts.
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