ArticleJournal of immunology (Baltimore, Md. : 1950)2015
Imaging of Cell-Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological Synapse and Novel PD-1 Dynamics.
Article in Journal of immunology (Baltimore, Md. : 1950), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
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- Automated detection of apoptotic bodies and cells in label-free time-lapse high-throughput video microscopy using deep convolutional neural networks.Bioinformatics (Oxford, England) · 2023Article
- Visualizing cell-cell communication using synthetic notch activated MRI.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Functionalized Lipid Droplets and Microfluidics Approach to Study Immune Cell Polarity In Vitro.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer.Methods in cell biology · 2023Article
- Cell pairing for biological analysis in microfluidic devices.Biomicrofluidics · 2022Review
- Phenotyping polarization dynamics of immune cells using a lipid droplet-cell pairing microfluidic platform.Cell reports methods · 2022Article
- Adhering interacting cells to two opposing coverslips allows super-resolution imaging of cell-cell interfaces.Communications biology · 2021Article
- Microfabricated Devices for Confocal Microscopy on Biological Samples.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Role of Actin Cytoskeleton Reorganization in Polarized Secretory Traffic at the Immunological Synapse.Frontiers in cell and developmental biology · 2021Review
- Microfluidics-Based Single-Cell Research for Intercellular Interaction.Frontiers in cell and developmental biology · 2021Review
- Shedding Structured Light on Molecular Immunity: The Past, Present and Future of Immune Cell Super Resolution Microscopy.Frontiers in immunology · 2021Review
- A novel CD2 staining-based flow cytometric assay for assessment of natural killer cell cytotoxicity.Journal of clinical laboratory analysis · 2020Article
- The Role of Immunological Synapse in Predicting the Efficacy of Chimeric Antigen Receptor (CAR) Immunotherapy.Cell communication and signaling : CCS · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The immunological synapse (IS) is one of the most pivotal communication strategies in immune cells. Understanding the molecular basis of the IS provides critical information regarding how immune cells mount an effective immune response. Fluorescence microscopy provides a fundamental tool to study the IS. However, current imaging techniques for studying the IS cannot sufficiently achieve high resolution in real cell-cell conjugates. In this study, we present a new device that allows for high-resolution imaging of the IS with conventional confocal microscopy in a high-throughput manner. Combining micropits and single-cell trap arrays, we have developed a new microfluidic platform that allows visualization of the IS in vertically "stacked" cells. Using this vertical cell pairing (VCP) system, we investigated the dynamics of the inhibitory synapse mediated by an inhibitory receptor, programed death protein-1, and the cytotoxic synapse at the single-cell level. In addition to the technique innovation, we have demonstrated novel biological findings by this VCP device, including novel distribution of F-actin and cytolytic granules at the IS, programed death protein-1 microclusters at the NK IS, and kinetics of cytotoxicity. We propose that this high-throughput, cost-effective, easy-to-use VCP system, along with conventional imaging techniques, can be used to address a number of significant biological questions in a variety of disciplines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.