ArticlePlant cell reports2015
FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Unambiguous chromosome identification by multicolour fluorescence in situ hybridisation reveals the genome organisation and evolution of sheepgrass (Leymus chinensis).BMC plant biology · 2026Article
- Satellite DNA inPlants (Basel, Switzerland) · 2025Article
- New insights into interspecies relationships, chromosomal evolution, and hybrid identification in the Lycoris Herb.BMC plant biology · 2025Article
- ND-FISH with New Oligo Probes for Chromosome Identification ofPlants (Basel, Switzerland) · 2024Article
- The Application of Fluorescence In Situ Hybridization in the Prescreening ofPlants (Basel, Switzerland) · 2024Article
- Obtainment and confirmation of intergeneric hybrids between marguerite (Plant biotechnology (Tokyo, Japan) · 2023Article
- All nonhomologous chromosomes and rearrangements inFrontiers in plant science · 2023Article
- Accurate Chromosome Identification in the Prunus SubgenusInternational journal of molecular sciences · 2022Article
- Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved.Plants (Basel, Switzerland) · 2022Review
- Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes inInternational journal of molecular sciences · 2022Article
- A chromosome-level, haplotype-phased Vanilla planifolia genome highlights the challenge of partial endoreplication for accurate whole-genome assembly.Plant communications · 2022Article
- Molecular Karyotyping onInternational journal of molecular sciences · 2021Article
- Genetic assessment of the effects of self-fertilization in aSaudi journal of biological sciences · 2021Article
- Creation of fertility-restored materials for Ogura CMS in Brassica oleracea by introducing Rfo gene from Brassica napus via an allotriploid strategy.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Genetic Characterization of an Endangered Chilean Endemic Species,Plants (Basel, Switzerland) · 2020Article
- Cytogenetic analysis of Bienertia sinuspersici Akhani as the first step in genome sequencing.Genes & genomics · 2020Article
- Identification of ribosomal sites and karyotype analysis in Festuca ulochaeta Steud. and Festuca fimbriata Ness., grasses native to Brazil.Molecular biology reports · 2020Article
- Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH).Genetics and molecular biology · 2020Article
- Production and First Assessment of Iranian Secondary Tritipyrum Genotypes by GISH and AFLP Markers.Iranian journal of biotechnology · 2019Article
- The story of promiscuous crucifers: origin and genome evolution of an invasive species, Cardamine occulta (Brassicaceae), and its relatives.Annals of botany · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe innovations in chromosome engineering have improved the efficiency of interrogation breeding, and the identification and transfer of resistance genes from alien to native species. Recent advances in molecular biology and cytogenetics have brought revolutionary, conceptual developments in mitosis and meiosis research, chromosome structure and manipulation, gene expression and regulation, and gene silencing. Cytogenetic studies offer integrative tools for imaging, genetics, epigenetics, and cytological information that can be employed to enhance chromosome and molecular genomic research in plant taxa. In situ hybridization techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), can identify chromosome morphologies and sequences, amount and distribution of various types of chromatin in chromosomes, and genome organization during the metaphase stage of meiosis. Over the past few decades, various new molecular cytogenetic applications have been developed. The FISH and GISH techniques present an authentic model for analyzing the individual chromosome, chromosomal segments, or the genomes of natural and artificial hybrid plants. These have become the most reliable techniques for studying allopolyploids, because most cultivated plants have been developed through hybridization or polyploidization. Moreover, introgression of the genes and chromatin from the wild types into cultivated species can also be analyzed. Since hybrid derivatives may have variable alien chromosome numbers or chromosome arms, the use of these approaches opens new avenues for accurately identifying genome differences.
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