ArticleMethods (San Diego, Calif.)2015
A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics.
Article in Methods (San Diego, Calif.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Breaking the Diffraction Limit: Progress and Prospects of Super-Resolution Fluorescence Microscopy in Biomedical and Materials Research.Journal of fluorescence · 2026Review
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
- Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique.The Journal of biological chemistry · 2021Review
- Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems.Pharmaceutics · 2021Review
- Article
- Background Reduction in STED-FCS Using a Bivortex Phase Mask.ACS photonics · 2020Article
- z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics.Biophysical journal · 2020Article
- The cortical actin network regulates avidity-dependent binding of hyaluronan by the lymphatic vessel endothelial receptor LYVE-1.The Journal of biological chemistry · 2020Article
- Impact of Nanoscale Hindrances on the Relationship between Lipid Packing and Diffusion in Model Membranes.The journal of physical chemistry. B · 2020Article
- HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly.Science advances · 2019Article
- Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS.Nature protocols · 2019Article
- Reconstitution of immune cell interactions in free-standing membranes.Journal of cell science · 2018Article
- Article
- Applications of STED fluorescence nanoscopy in unravelling nanoscale structure and dynamics of biological systems.Journal of biosciences · 2018Review
- The Diverse Range of Possible Cell Membrane Interactions with Substrates: Drug Delivery, Interfaces and Mobility.Molecules (Basel, Switzerland) · 2017Review
- Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state.Nature communications · 2017Article
- Super-resolution optical microscopy for studying membrane structure and dynamics.Journal of physics. Condensed matter : an Institute of Physics journal · 2017Review
- Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS.Nature communications · 2017Article
- Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.Molecular biology of the cell · 2017Article
- Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS.Journal of physics D: Applied physics · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with fluorescence correlation spectroscopy (FCS). STED-FCS combines the diffraction-unlimited spatial resolution of STED microscopy with the statistical accuracy of FCS to determine sub-millisecond-fast molecular dynamics with single-molecule sensitivity. A unique advantage of STED-FCS is that the observation spot for the FCS data recordings can be tuned to sub-diffraction scales, i.e. <200 nm in diameter, in a gradual manner to investigate fast diffusion of membrane-incorporated labelled entities. Unfortunately, so far the STED-FCS technology has mostly been applied on a few custom-built setups optimised for far-red fluorescent emitters. Here, we summarise the basics of the STED-FCS technology and highlight how it can give novel details into molecular diffusion modes. Most importantly, we present a straightforward way for performing STED-FCS measurements on an unmodified turnkey commercial system using a time-gated detection scheme. Further, we have evaluated the STED-FCS performance of different commonly used green emitting fluorescent dyes applying freely available, custom-written analysis software.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.