ArticleScientific reports2015
Enzymatic measurement of phosphatidylglycerol and cardiolipin in cultured cells and mitochondria.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
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- Chikungunya Replication and Infection Is Dependent upon and Alters Cellular Hexosylceramide Levels in Vero Cells.Viruses · 2025Article
- A New Perspective on the Role of Alterations in Mitochondrial Proteins Involved in ATP Synthesis and Mobilization in Cardiomyopathies.International journal of molecular sciences · 2025Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Phospholipid biomarkers of coronary heart disease.Journal of pharmaceutical health care and sciences · 2024Review
- Lipidomic landscape of lipokines in adipose tissue derived extracellular vesicles.Frontiers in molecular biosciences · 2023Article
- Staphylococcal phosphatidylglycerol antigens activate human T cells via CD1a.Nature immunology · 2023Article
- Mitochondrial Fission Process 1 controls inner membrane integrity and protects against heart failure.Nature communications · 2022Article
- Determination of Glycerophospholipids in Biological Material Using High-Performance Liquid Chromatography with Charged Aerosol Detector HPLC-CAD-A New Approach for Isolation and Quantification.Molecules (Basel, Switzerland) · 2022Article
- A Peptide-Based Fluorescent Sensor for Anionic Phospholipids.ACS omega · 2022Article
- Comparisons of High Intensity Interval Training and Continuous Training on Metabolomic Alteration and Cardiac Function in Male Adolescent Rats.Frontiers in physiology · 2022Article
- Fat of the Gut: Epithelial Phospholipids in Inflammatory Bowel Diseases.International journal of molecular sciences · 2021Review
- Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Environmental science & technology · 2021Article
- Novel Molecular Insights into Human Lipid-Mediated T Cell Immunity.International journal of molecular sciences · 2021Review
- Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylglycerol (PG) and cardiolipin (CL) are synthesized in mitochondria and regulate numerous biological functions. In this study, a novel fluorometric method was developed for measuring PG and CL using combinations of specific enzymes and Amplex Red. This assay quantified the sum of PG and CL (PG + CL) regardless of the species of fatty acyl chain. The calibration curve for PG + CL measurement was linear, and the detection limit was 1 μM (10 pmol in the reaction mixture). This new method was applied to the determinations of PG + CL content in HEK293 cells and CL content in purified mitochondria, because the mitochondrial content of PG is negligible compared with that of CL. We demonstrated that the PG+CL content was greater at low cell density than at high cell density. The overexpression of phosphatidylglycerophosphate synthase 1 (PGS1) increased the cellular contents of PG + CL and phosphatidylcholine (PC), and reduced that of phosphatidic acid. PGS1 overexpression also elevated the mitochondrial contents of CL and PC, but had no effect on the number of mitochondria per cell. In addition to the enzymatic measurements of other phospholipids, this simple, sensitive and high-throughput assay for measuring PG + CL can be used to understand cellular, physiological and pathological processes.
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