ArticleMicrovascular research2015
Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices.
Article in Microvascular research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.
- Human endothelial colony-forming cells in regenerative therapy: A systematic review of controlled preclinical animal studies.Stem cells translational medicine · 2020Pooled it
- Standardization of methods to quantify and culture endothelial colony-forming cells derived from peripheral blood: Position paper from the International Society on Thrombosis and Haemostasis SSC.Journal of thrombosis and haemostasis : JTH · 2019Guideline
- Evaluation of Platelet Lysate-Based Medium and Protein Substrate for HUVEC Culture and Expansion.Biomedicines · 2025Article
- Human Endothelial Colony-Forming Cells.Cold Spring Harbor perspectives in medicine · 2022Article
- Therapeutic Potential of Endothelial Colony-Forming Cells in Ischemic Disease: Strategies to Improve their Regenerative Efficacy.International journal of molecular sciences · 2020Review
- Article
- Injectable Highly Tunable Oligomeric Collagen Matrices for Dental Tissue Regeneration.ACS applied bio materials · 2020Article
- Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells.Molecular medicine reports · 2019Article
- Cord Blood Platelet Lysate: In Vitro Evaluation to Support the Use in Regenerative Medicine.Mediterranean journal of hematology and infectious diseases · 2019Article
- Endoglin as an Adhesion Molecule in Mature and Progenitor Endothelial Cells: A Function Beyond TGF-β.Frontiers in medicine · 2019Review
- 3D matrix-embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells.Journal of tissue engineering and regenerative medicine · 2018Article
- The Vasoreparative Potential of Endothelial Colony Forming Cells: A Journey Through Pre-clinical Studies.Frontiers in medicine · 2018Review
- Recent Advances in Endothelial Colony Forming Cells Toward Their Use in Clinical Translation.Frontiers in medicine · 2018Review
- Tissue regeneration using endothelial colony-forming cells: promising cells for vascular repair.Pediatric research · 2018Review
- Endothelial Progenitors: A Consensus Statement on Nomenclature.Stem cells translational medicine · 2017Review
- Akt isoform specific effects in ovarian cancer progression.Oncotarget · 2016Article
- Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Human cord blood (CB) is enriched in circulating endothelial colony forming cells (ECFCs) that display high proliferative potential and in vivo vessel forming ability. Since diminished ECFC survival is known to dampen the vasculogenic response in vivo, we tested how long implanted ECFC survive and generate vessels in three-dimensional (3D) type I collagen matrices in vitro and in vivo. We hypothesized that human platelet lysate (HPL) would promote cell survival and enhance vasculogenesis in the 3D collagen matrices. We report that the percentage of ECFC co-cultured with HPL that were alive was significantly enhanced on days 1 and 3 post-matrix formation, compared to ECFC alone containing matrices. Also, co-culture of ECFC with HPL displayed significantly more vasculogenic activity compared to ECFC alone and expressed significantly more pro-survival molecules (pAkt, p-Bad and Bcl-xL) in the 3D collagen matrices in vitro. Treatment with Akt1 inhibitor (A-674563), Akt2 inhibitor (CCT128930) and Bcl-xL inhibitor (ABT-263/Navitoclax) significantly decreased the cell survival and vasculogenesis of ECFC co-cultured with or without HPL and implicated activation of the Akt1 pathway as the critical mediator of the HPL effect on ECFC in vitro. A significantly greater average vessel number and total vascular area of human CD31(+) vessels were present in implants containing ECFC and HPL, compared to the ECFC alone implants in vivo. We conclude that implantation of ECFC with HPL in vivo promotes vasculogenesis and augments blood vessel formation via diminishing apoptosis of the implanted ECFC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.