ArticleJournal of the American Society for Mass Spectrometry2015
Improving the Sensitivity of Matrix-Assisted Ionization (MAI) Mass Spectrometry Using Ammonium Salts.
Article in Journal of the American Society for Mass Spectrometry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- The Role of Aerosol Liquid Water in Droplet-Assisted Ionization Mass Spectrometry.Analytical chemistry · 2025Article
- New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.Journal of the American Society for Mass Spectrometry · 2024Review
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Instrumentation development, improvement, simplification, and miniaturization: The multifunctional plate source for use in mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2023Article
- Applications and continued evolution of glycan imaging mass spectrometry.Mass spectrometry reviews · 2023Review
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- MALDI-TOF mass spectrometry-based quantification of C-peptide in diabetes patients.European journal of mass spectrometry (Chichester, England) · 2020Article
- Spontaneous Charge Separation and Sublimation Processes are Ubiquitous in Nature and in Ionization Processes in Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Matrix-Assisted Ionization on a Portable Mass Spectrometer: Analysis Directly from Biological and Synthetic Materials.Analytical chemistry · 2016Article
- High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- "Magic" Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
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Authors and funding
2 authors.
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Abstract
In matrix-assisted ionization (MAI), analyte incorporated in a small molecule matrix is introduced into an aperture linking atmospheric pressure with the vacuum of a mass spectrometer. Gas-phase analyte ions are spontaneously produced without use of a laser or high voltage. Here we investigate analyte and background ion abundances upon addition of ammonium salts to various MAI matrix/analyte solutions. Regardless of the ammonium salt or matrix used, chemical background ions are suppressed and/or analyte ion abundance improved for basic small molecules, peptides, and proteins. Background ion abundances increase with increasing inlet temperature, but are suppressed with addition of any of a variety of ammonium salts without much effect on the total ion abundances of the analyte ions. However, at lower inlet temperature using the matrices 2-bromo-2-nitropropane-1,3-diol, 1,2-dicyanobenzene, and 3-nitrobenzonitrile (3-NBN), analyte ion abundance increases and any chemical background decreases upon addition of ammonium salt. The improvement in sensitivity using 3-NBN with ammonium salt allows full acquisition mass spectra consuming as little as 1 fmol of ubiquitin. More complete peptide coverage for 100 fmol of a BSA tryptic digest, and increased sensitivity of drugs spiked in urine and saliva were observed after ammonium salt addition to the 3-NBN matrix.
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