Evidence map›Paper›PMID 26122505›Full record

ArticleAllergy, asthma & immunology research2015

Altered microRNA Expression Profiles of Extracellular Vesicles in Nasal Mucus From Patients With Allergic Rhinitis.

Geping Wu, Guanghai Yang, Ruxin Zhang, Guangyin Xu, Ling Zhang, Wu Wen, Jianbing Lu, Jianyong Liu, Yan Yu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Allergy, asthma & immunology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 3 pooled it
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 3 syntheses or guidelines pooled it, 98 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Roles of Exosomes in Chronic Rhinosinusitis: A Systematic Review.International journal of molecular sciences · 2022
    Pooled it
  4. Article
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  6. Review
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  8. Article
  9. Review
  10. Article
  11. Article
  12. Functions and Clinical Applications of Extracellular Vesicles in TInternational journal of molecular sciences · 2024
    Review
  13. Article
  14. Review
  15. Review
  16. Article
  17. Article
  18. Current Insights on the Impact of Proteomics in Respiratory Allergies.International journal of molecular sciences · 2022
    Review
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Geping WuDepartment of Otolaryngology, First People Hospital of Zhangjiagang City, Suzhou, China. Gordon-wu@qq.com.
Guanghai YangDepartment of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Ruxin ZhangDepartment of Otolaryngology, Huadong Hospital, Fudan University, Shanghai, China.
Guangyin XuInstitute of Neuroscience and Department of Neurobiology and Psychology, Key lab of Pain Research and Therapy, Soochow University, Suzhou, China.
Ling ZhangDepartment of Science and Education, First People Hospital of Zhangjiagang City, Suzhou, China.
Wu WenDepartment of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai, China.
Jianbing LuDepartment of Otolaryngology, First People Hospital of Zhangjiagang City, Suzhou, China.
Jianyong LiuDepartment of Otolaryngology, First People Hospital of Zhangjiagang City, Suzhou, China.
Yan YuDepartment of Otolaryngology, First People Hospital of Zhangjiagang City, Suzhou, China.
Soochow University · CNHuadong Hospital · CNHuazhong University of Science and Technology · CNSecond Military Medical University · CNZhangjiagang First People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeAllergic rhinitis (AR) is an inflammatory disorder of the upper airway. Exosomes or extracellular vesicles are nanosized vesicles of endosomal origin released from inflammatory and epithelial cells that have been implicated in allergic diseases. In this study, we characterized the microRNA (miRNA) content of exosomes in AR.

methodsExtracellular vesicles were isolated from nasal mucus from healthy control subjects (n=10) and patients with severe AR (n=10). Vesicle RNA was analyzed by using a TaqMan microRNA assays Human Panel-Early Access kit (Applied Biosystems, Foster City, CA, USA) containing probes for 366 human miRNAs, and selected findings were validated with quantitative RT-PCR. Target prediction and pathway analysis for the differentially expressed miRNAs were performed using DIANA-mirPath.

resultsTwenty-one vesicle miRNAs were up-regulated and 14 miRNAs were under-regulated significantly (P<0.05) in nasal mucus from AR patients when compared to healthy controls. Bioinformatic analysis by DIANA-mirPath demonstrated that 32 KEGG biological processes were significantly enriched (P<0.05, FDR corrected) among differentially expressed vesicle miRNA signatures. Among them, the B-cell receptor signaling pathway (P=3.709E-09), the natural killer cell-mediated cytotoxicity (P=8.466E-05), the T-cell receptor signaling pathway (P=0.00075), the RIG-I-like receptor signaling pathway (P=0.00127), the Wnt signaling pathway (P=0.00130), endocytosis (P=0.00440), and salivary secretion (P=0.04660) were the most prominent pathways enriched in quantiles with differential vesicle miRNA patterns. Furthermore, miR-30-5p, miR-199b-3p, miR-874, miR-28-3p, miR-203, and miR-875-5p, involved in B-cell receptor and salivary secretion signaling pathways, were selected for validation using independent samples from 44 AR patients and 20 healthy controls. MiR-30-5p and miR-199b-3p were significantly increased in extracellular vesicles from nasal mucus when compared to healthy controls, while miR-874 and miR-28-3p were significantly down-regulated. In addition, miRNA-203 was significantly increased in AR patients, while miRNA-875-5p was found to be significantly decreased in AR patients.

conclusionsThis study demonstrated that vesicle miRNA may be a regulator for the development of AR.

Indexed as

allergic rhinitisExtracellular vesiclesmicroRNAs

Identifiers

PMID26122505
PMCPMC4509657
OpenAlexW824207155

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.