ArticleScientific reports2015
Whole genome amplification with SurePlex results in better copy number alteration detection using sequencing data compared to the MALBAC method.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Performance Evaluation of Whole-Genome Amplification Platforms for Clinical Next-Generation Sequencing with Minimal Nucleic Acid Input.Annals of laboratory medicine · 2026Article
- Whole Genomic Amplification for Detecting Single-Gene Variants Causing Inherited Disease.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Changes in sequencing technology used for preimplantation genetic testing for aneuploidy.Journal of assisted reproduction and genetics · 2025Review
- Translocation-specific polymerase chain reaction in preimplantation genetic testing for recurrent translocation carrier.Journal of human genetics · 2025Article
- Differential performance of strategies for single-cell whole-genome amplification.Cell reports methods · 2025Article
- The importance of standardizing criteria for PGT-A interpretation of blastocysts analyzed by next-generation sequencing.JBRA assisted reproduction · 2023Article
- Non-invasive preimplantation genetic testing: a literature review.JBRA assisted reproduction · 2022Review
- New Perspectives for Whole Genome Amplification in Forensic STR Analysis.International journal of molecular sciences · 2022Review
- Recent advances and application of whole genome amplification in molecular diagnosis and medicine.MedComm · 2022Review
- Karyotype of the Blastocoel Fluid Derived by Laser-Assisted Hatching Demonstrates a Low Agreement With the Trophectoderm.Frontiers in physiology · 2022Article
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- Uncovering low-level mosaicism in human embryonic stem cells using high throughput single cell shallow sequencing.Scientific reports · 2019Article
- Human Brain Single Nucleotide Polymorphism: Validation of DNA Sequencing.Journal of Alzheimer's disease reports · 2018Review
- Systematic assessment of the performance of whole-genome amplification for SNP/CNV detection and β-thalassemia genotyping.Journal of human genetics · 2018Article
- The performance of MALBAC and MDA methods in the identification of concurrent mutations and aneuploidy screening to diagnose beta-thalassaemia disorders at the single- and multiple-cell levels.Journal of clinical laboratory analysis · 2018Article
- Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells.Scientific reports · 2018Article
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- Novel Double Factor PGT strategy analyzing blastocyst stage embryos in a single NGS procedure.PloS one · 2018Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current whole genome amplification (WGA) methods lead to amplification bias resulting in over- and under-represented regions in the genome. Nevertheless, certain WGA methods, such as SurePlex and subsequent arrayCGH analysis, make it possible to detect copy number alterations (CNAs) at a 10 Mb resolution. A more uniform WGA combined with massive parallel sequencing (MPS), however, could allow detection at higher resolution and lower cost. Recently, MALBAC, a new WGA method, claims unparalleled performance. Here, we compared the well-established SurePlex and MALBAC WGA for their ability to detect CNAs in MPS generated data and, in addition, compared PCR-free MPS library preparation with the standard enrichment PCR library preparation. Results showed that SurePlex amplification led to more uniformity across the genome, allowing for a better CNA detection with less false positives compared to MALBAC amplified samples. An even more uniform coverage was observed in samples following a PCR-free library preparation. In general, the combination of SurePlex and MPS led to the same chromosomal profile compared to a reference arrayCGH from unamplified genomic DNA, underlining the large potential of MPS techniques in CNA detection from a limited number of DNA material.
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