ArticleCurrent gene therapy2015
A Simple High Efficiency Intra-Islet Transduction Protocol Using Lentiviral Vectors.
Article in Current gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators.bioRxiv : the preprint server for biology · 2025Article
- Roles for the long non-coding RNAiScience · 2025Article
- Cell Therapies and Gene Therapy for Diabetes: Current Progress.Current diabetes reviews · 2025Review
- RNA-binding protein PCBP2 regulates pancreatic β cell function and adaptation to glucose.The Journal of clinical investigation · 2024Article
- PIMT Controls Insulin Synthesis and Secretion through PDX1.International journal of molecular sciences · 2023Article
- Metabolic reprogramming by Acly inhibition using SB-204990 alters glucoregulation and modulates molecular mechanisms associated with aging.Communications biology · 2023Article
- β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130.Nature metabolism · 2022Article
- β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications.International journal of molecular sciences · 2021Review
- IL-10 lentivirus-laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury.Biotechnology and bioengineering · 2021Article
- Ins1-Cre and Ins1-CreER Gene Replacement Alleles Are Susceptible To Silencing By DNA Hypermethylation.Endocrinology · 2020Article
- Inadequate control of thyroid hormones sensitizes to hepatocarcinogenesis and unhealthy aging.Aging · 2019Article
- JUND regulates pancreatic β cell survival during metabolic stress.Molecular metabolism · 2019Article
- Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates.Journal of visualized experiments : JoVE · 2019Article
- A PDX1-ATF transcriptional complex governs β cell survival during stress.Molecular metabolism · 2018Article
- The type 2 diabetes-associated HMG20A gene is mandatory for islet beta cell functional maturity.Cell death & disease · 2018Article
- Levothyroxine enhances glucose clearance and blunts the onset of experimental type 1 diabetes mellitus in mice.British journal of pharmacology · 2017Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Successful normalization of blood glucose in patients transplanted with pancreatic islets isolated from cadaveric donors established the proof-of-concept that Type 1 Diabetes Mellitus is a curable disease. Nonetheless, major caveats to the widespread use of this cell therapy approach have been the shortage of islets combined with the low viability and functional rates subsequent to transplantation. Gene therapy targeted to enhance survival and performance prior to transplantation could offer a feasible approach to circumvent these issues and sustain a durable functional β-cell mass in vivo. However, efficient and safe delivery of nucleic acids to intact islet remains a challenging task. Here we describe a simple and easy-to-use lentiviral transduction protocol that allows the transduction of approximately 80 % of mouse and human islet cells while preserving islet architecture, metabolic function and glucose-dependent stimulation of insulin secretion. Our protocol will facilitate to fully determine the potential of gene expression modulation of therapeutically promising targets in entire pancreatic islets for xenotransplantation purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.