Evidence map›Paper›PMID 26122086›Full record

ArticleBMC genomics2015

A user-friendly workflow for analysis of Illumina gene expression bead array data available at the arrayanalysis.org portal.

Lars M T Eijssen, Varshna S Goelela, Thomas Kelder, Michiel E Adriaens, Chris T Evelo, Marijana Radonjic

Abstract read
In one paragraph

Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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  5. The Effects ofNutrients · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lars M T EijssenDepartment of Bioinformatics-BiGCaT, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands. l.eijssen@maastrichtuniversity.nl.
Varshna S GoelelaTNO, Research Group Microbiology & Systems Biology, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands. varshna.goelela@crl.com.
Thomas KelderTNO, Research Group Microbiology & Systems Biology, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands. thomas@edgeleap.com.
Michiel E AdriaensDepartment of Bioinformatics-BiGCaT, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands. m.e.adriaens@amc.uva.nl.
Chris T EveloDepartment of Bioinformatics-BiGCaT, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands. chris.evelo@maastrichtuniversity.nl.
Marijana RadonjicTNO, Research Group Microbiology & Systems Biology, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands. marijana@edgeleap.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIllumina whole-genome expression bead arrays are a widely used platform for transcriptomics. Most of the tools available for the analysis of the resulting data are not easily applicable by less experienced users. ArrayAnalysis.org provides researchers with an easy-to-use and comprehensive interface to the functionality of R and Bioconductor packages for microarray data analysis. As a modular open source project, it allows developers to contribute modules that provide support for additional types of data or extend workflows.

resultsTo enable data analysis of Illumina bead arrays for a broad user community, we have developed a module for ArrayAnalysis.org that provides a free and user-friendly web interface for quality control and pre-processing for these arrays. This module can be used together with existing modules for statistical and pathway analysis to provide a full workflow for Illumina gene expression data analysis. The module accepts data exported from Illumina's GenomeStudio, and provides the user with quality control plots and normalized data. The outputs are directly linked to the existing statistics module of ArrayAnalysis.org, but can also be downloaded for further downstream analysis in third-party tools.

conclusionsThe Illumina bead arrays analysis module is available at http://www.arrayanalysis.org . A user guide, a tutorial demonstrating the analysis of an example dataset, and R scripts are available. The module can be used as a starting point for statistical evaluation and pathway analysis provided on the website or to generate processed input data for a broad range of applications in life sciences research.

Indexed as

User-Computer InterfaceComputational BiologyInternetOligonucleotide Array Sequence AnalysisQuality Control

Identifiers

PMID26122086
PMCPMC4486126

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.