Evidence map›Paper›PMID 26122028›Full record

ArticleInternational journal of obesity (2005)2016

Obesity reduces the pro-angiogenic potential of adipose tissue stem cell-derived extracellular vesicles (EVs) by impairing miR-126 content: impact on clinical applications.

G Togliatto, P Dentelli, M Gili, S Gallo, C Deregibus, E Biglieri, A Iavello, E Santini, C Rossi, A Solini and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of obesity (2005), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed, 1 pooled it
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.

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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

G TogliattoDepartment of Medical Sciences, University of Torino, Torino, Italy.
P DentelliDepartment of Medical Sciences, University of Torino, Torino, Italy.
M GiliDepartment of Medical Sciences, University of Torino, Torino, Italy.
S GalloDepartment of Medical Sciences, University of Torino, Torino, Italy.
C DeregibusDepartment of Medical Sciences, University of Torino, Torino, Italy.
E BiglieriDepartment of Medical Sciences, University of Torino, Torino, Italy.
A IavelloDepartment of Medical Sciences, University of Torino, Torino, Italy.
E SantiniDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
C RossiDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
A SoliniDepartment of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
G CamussiDepartment of Medical Sciences, University of Torino, Torino, Italy.
M F BrizziDepartment of Medical Sciences, University of Torino, Torino, Italy.
University of Turin · ITUniversity of Pisa · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

objectivesSoluble factors and cell-derived extracellular vesicles (EVs) are crucial tissue repair mediators in cell-based therapy. In the present study, we investigate the therapeutic impact of EVs released by adipose tissue-derived stem cells (ASCs) recovered from obese subjects' visceral and subcutaneous tissues.

methodsASCs were recovered from 10 obese (oASCs) and 6 non-obese (nASCs) participants and characterized. In selected experiments, nASCs and oASCs were cultured with palmitic acid (PA) or high glucose (HG), respectively. EVs from obese (oEVs) and non-obese (nEVs) subjects' visceral and subcutaneous ASCs were collected after ultracentrifugation and analyzed for their cargo: microRNA-126 (miR-126), vascular endothelial growth factor (VEGF), and matrix metalloproteinase 2 (MMP-2), and for their biological effects on endothelial cells (ECs). Western blotting analysis and loss- and gain-of function experiments were performed.

resultsoEVs show impaired angiogenic potential compared with nEVs. This effect depends on EV cargo: reduced content of VEGF, MMP-2 and, more importantly, miR-126. We demonstrate, using gain- and loss-of-function experiments, that this reduced miR-126 content leads to Spred1 upregulation and the inhibition of the extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase pathway in ECs. We also show that PA treatment of nASCs translates into the release of EVs that recapitulate oEV cargo. Moreover, HG treatment of oASCs further reduces miR-126 EV content and EV-mediated in vitro angiogenesis. Finally, impaired pro-angiogenic potential is also detected in EVs released from obese subcutaneous adipose tissue-derived ASCs.

conclusionsThese results indicate that obesity impacts on EV pro-angiogenic potential and may raise concerns about the use of adipose tissue-derived EVs in cell-based therapy in the obese setting.

Indexed as

AdipogenesisAdult Stem CellsCell DifferentiationCells, CulturedEndothelial CellsExtracellular VesiclesHumansIntra-Abdominal FatMicroRNAsMicrovesselsObesitySubcutaneous FatVascular Endothelial Growth Factor AMicroRNAsMIRN126 microRNA, humanVascular Endothelial Growth Factor A

Identifiers

PMID26122028
PMCPMC4722244
OpenAlexW1533680245

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.