Evidence map›Paper›PMID 26120272›Full record

ArticleMolecular vision2015

Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy.

Leo A Kim, Lindsay L Wong, Dhanesh S Amarnani, Alexander A Bigger-Allen, Yang Hu, Christina K Marko, Dean Eliott, Vinay A Shah, Declan McGuone, Anat O Stemmer-Rachamimov and 3 more

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 23 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Article
  5. Article
  6. Ref-1 redox activity regulates retinal neovascularization by modulating transcriptional activation of HIF-1α.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
    Article
  7. Review
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  11. Article
  12. Article
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  15. Blood Brain Barrier Injury in Diabetes: Unrecognized Effects on Brain and Cognition.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2017
    Review
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Leo A KimSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA ; Retina Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Lindsay L WongSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Dhanesh S AmarnaniSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Alexander A Bigger-AllenSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Yang HuSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Christina K MarkoSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Dean EliottRetina Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Vinay A ShahDean McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK.
Declan McGuoneC.S. Kubik Laboratory for Neuropathology, Massachusetts General Hospital, Boston, MA.
Anat O Stemmer-RachamimovC.S. Kubik Laboratory for Neuropathology, Massachusetts General Hospital, Boston, MA.
Xiaowu GaiOcular Genomics Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA.
Patricia A D'AmoreSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA ; Department of Pathology, Harvard Medical School, Boston, MA.
Joseph F Arboleda-VelasquezSchepens Eye Research Institute/Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA.
Massachusetts Eye and Ear Infirmary · USMassachusetts General Hospital · USDean McGee Eye Institute · US

Funding

Mass Eye and Ear-Vision Clinical Scientist Development ProgramK12EY016335 · NEI · MASSACHUSETTS EYE AND EAR INFIRMARY · PI Reza Dana · 2004 to 2026
$12.3M
THE ROLE OF MICROVASCULAR CELLS IN NEOVASCULARIZATIONR01EY005318 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI D'AMORE, PATRICIA ANN · 1985 to 2015
$6.4M
Notch and TGF-beta in the Control of Retinal Vascular IntegrityR00EY021624 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI ARBOLEDA-VELASQUEZ, JOSEPH · 2014 to 2016
$736k
NEI NIH HHS K12 EY016335NEI NIH HHS K12EY16335NEI NIH HHS R00 EY021624NEI NIH HHS R00EY021624NEI NIH HHS R01 EY005318NEI NIH HHS R01EY005318
6 · The paper itself

Abstract

purposeEpiretinal fibrovascular membranes (FVMs) are a hallmark of proliferative diabetic retinopathy (PDR). Surgical removal of FVMs is often indicated to treat tractional retinal detachment. This potentially informative pathological tissue is usually disposed of after surgery without further examination. We developed a method for isolating and characterizing cells derived from FVMs and correlated their expression of specific markers in culture with that in tissue.

methodsFVMs were obtained from 11 patients with PDR during diabetic vitrectomy surgery and were analyzed with electron microscopy (EM), comparative genomic hybridization (CGH), immunohistochemistry, and/or digested with collagenase II for cell isolation and culture. Antibody arrays and enzyme-linked immunosorbent assay (ELISA) were used to profile secreted angiogenesis-related proteins in cell culture supernatants.

resultsEM analysis of the FVMs showed abnormal vessels composed of endothelial cells with large nuclei and plasma membrane infoldings, loosely attached perivascular cells, and stromal cells. The cellular constituents of the FVMs lacked major chromosomal aberrations as shown with CGH. Cells derived from FVMs (C-FVMs) could be isolated and maintained in culture. The C-FVMs retained the expression of markers of cell identity in primary culture, which define specific cell populations including CD31-positive, alpha-smooth muscle actin-positive (SMA), and glial fibrillary acidic protein-positive (GFAP) cells. In primary culture, secretion of angiopoietin-1 and thrombospondin-1 was significantly decreased in culture conditions that resemble a diabetic environment in SMA-positive C-FVMs compared to human retinal pericytes derived from a non-diabetic donor.

conclusionsC-FVMs obtained from individuals with PDR can be isolated, cultured, and profiled in vitro and may constitute a unique resource for the discovery of cell signaling mechanisms underlying PDR that extends beyond current animal and cell culture models.

Indexed as

ActinsAdultAngiopoietin-1Cell ProliferationCells, CulturedCell SeparationComparative Genomic HybridizationDiabetic RetinopathyEpiretinal MembraneFemaleGlial Fibrillary Acidic ProteinHumansImmunohistochemistryMaleMiddle AgedPlatelet Endothelial Cell Adhesion Molecule-1ACTA2 protein, humanActinsAngiopoietin-1ANGPT1 protein, humanGlial Fibrillary Acidic ProteinPlatelet Endothelial Cell Adhesion Molecule-1

Identifiers

PMID26120272
PMCPMC4462955
OpenAlexW598252718

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.