ArticleEnvironmental microbiology2016
Let there be bioluminescence: development of a biophotonic imaging platform for in situ analyses of oral biofilms in animal models.
Article in Environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Identification of a conserved GNAT-family lysine acetyltransferase inJournal of bacteriology · 2026Article
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- Drug repurposing for glucosyltransferase inhibition for targeted oral biofilm disruption.NPJ biofilms and microbiomes · 2026Article
- Marginal integrity produced by quaternary ammonium methacrylate-based dental adhesive tested under physiologically relevant models.Dental materials : official publication of the Academy of Dental Materials · 2025Article
- Cell wall glycosyltransferase ofInfection and immunity · 2025Article
- Development of SacB-based counterselection for efficient allelic exchange inMicrobiology spectrum · 2025Article
- Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms.Dental materials : official publication of the Academy of Dental Materials · 2024Article
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome ofMicrobiology spectrum · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Development of a xylose-inducible promoter and riboswitch combination system for manipulating gene expression inApplied and environmental microbiology · 2023Article
- The potential use of glycosyl-transferase inhibitors for targeted reduction of S. mutans biofilms in dental materials.Scientific reports · 2023Article
- Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular ecological studies.FEMS microbiology reviews · 2023Review
- Triacrylamide-Based Adhesives Stabilize Bonds in Physiologic Conditions.Journal of dental research · 2022Article
- Development of the First Tractable Genetic System for Parvimonas micra, a Ubiquitous Pathobiont in Human Dysbiotic Disease.Microbiology spectrum · 2022Article
- Genomic and phenotypic characterization ofFrontiers in microbiology · 2022Article
- The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Regulatory control of the Streptococcus mutans HdrRM LytTR Regulatory System functions via a membrane sequestration mechanism.Molecular microbiology · 2020Article
- Effect of biofilm exposure on marginal integrity of composite restorations.American journal of dentistry · 2020Article
- Magnesium-Dependent Promotion of HJournal of dental research · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
In the current study, we describe a novel biophotonic imaging-based reporter system that is particularly useful for the study of virulence in polymicrobial infections and interspecies interactions within animal models. A suite of luciferase enzymes was compared using three early colonizing species of the human oral flora (Streptococcus mutans, Streptococcus gordonii and Streptococcus sanguinis) to determine the utility of the different reporters for multiplexed imaging studies in vivo. Using the multiplex approach, we were able to track individual species within a dual-species oral infection model in mice with both temporal and spatial resolution. We also demonstrate how biophotonic imaging of multiplexed luciferase reporters could be adapted for real-time quantification of bacterial gene expression in situ. By creating an inducible dual-luciferase expressing reporter strain of S. mutans, we were able to exogenously control and measure expression of nlmAB (encoding the bacteriocin mutacin IV) within mice to assess its importance for the persistence ability of S. mutans in the oral cavity. The imaging system described in the current study circumvents many of the inherent limitations of current animal model systems, which should now make it feasible to test hypotheses that were previously impractical to model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.