Evidence map›Paper›PMID 26118995›Full record

ArticleCell host & microbe2015

Global Mapping of the Inc-Human Interactome Reveals that Retromer Restricts Chlamydia Infection.

Kathleen M Mirrashidi, Cherilyn A Elwell, Erik Verschueren, Jeffrey R Johnson, Andrew Frando, John Von Dollen, Oren Rosenberg, Natali Gulbahce, Gwendolyn Jang, Tasha Johnson and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

141 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Live Imaging of Ions in Cells Infected with Chlamydia trachomatis.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  3. Article
  4. Inclusion Membrane Proteins ofMicroorganisms · 2026
    Review
  5. Article
  6. Sorting nexin 5 mediates antigen presentation and immunity againstbioRxiv : the preprint server for biology · 2026
    Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. ThebioRxiv : the preprint server for biology · 2025
    Article
  13. Article
  14. Pathogen-induced rerouting of host membrane trafficking.Current opinion in cell biology · 2025
    Review
  15. Article
  16. A disease-specific convergence of host and Epstein-Barr virus genetics in multiple sclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  17. Journal of bacteriology · 2025
    Review
  18. Article
  19. Article
  20. Article

81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Kathleen M MirrashidiDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Cherilyn A ElwellDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Erik VerschuerenQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Jeffrey R JohnsonQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Andrew FrandoDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
John Von DollenQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Oren RosenbergDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Natali GulbahceQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Gwendolyn JangQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Tasha JohnsonQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Stefanie JägerQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Anusha M GopalakrishnanDepartment of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
Jessica SherryDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Joe Dan DunnDepartment of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
Andrew OliveDepartment of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
Bennett PennDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Michael ShalesDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Gladstone Institutes, San Francisco, CA 94158, USA.
Jeffery S CoxDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
Michael N StarnbachDepartment of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
Isabelle DerreDepartment of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT 06510, USA.
Raphael ValdiviaDepartment of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
Nevan J KroganQB3, California Institute for Quantitative Biosciences, San Francisco, CA 94148, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address: nevan.krogan@ucsf.edu.
Joanne EngelDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: jengel@medicine.ucsf.edu.

Funding

Virology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STEVEN Grant DEEKS · 1988 to 2026
$93.6M
X-ray Screening and Rapid Structure DeterminationP50GM082250 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FRANKEL, ALAN D · 2007 to 2018
$48.8M
The Burden of Chikungunya and Dengue Infection in KenyaU54AI081680 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI RYMAN, KATHERINE D · 2009 to 2013
$40.4M
Virus CoreU19AI106754 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI ALBRECHT, RANDY A. · 2013 to 2017
$26.0M
Molecular Virology and Systems Biology Screening CoreP01AI090935 · NIAID · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI KOENIG, RENATE · 2010 to 2014
$20.4M
Rewiring Therapeutic Cells: User-Controlled T Cells and Stem CellsP50GM081879 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIM, WENDELL A · 2010 to 2017
$19.0M
Cytotoxic T Cell Mediated Immunity to ChlamydiaR01AI039558 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI STARNBACH, MICHAEL N · 2001 to 2025
$15.6M
The EPI-STI Biostatistics and Bioinformatics Core (Cuzick/Wheeler)U19AI113187 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI WHEELER, COSETTE MARIE · 2014 to 2018
$12.6M
MOLECULAR AND CELLULAR IMMUNOLOGYT32AI007334 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CYSTER, JASON G · 1988 to 2023
$11.0M
Novel approaches to identify host genes required for Chlamydia pathogenesisR01AI073770 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ENGEL, JOANNE N. · 2007 to 2016
$3.8M
Forward and Reverse Genetics in ChlamydiaR01AI100759 · NIAID · DUKE UNIVERSITY · PI VALDIVIA, RAPHAEL H · 2012 to 2016
$2.2M
ER-Chlamydia Inclusion Membrane Contact SitesR01AI101441 · NIAID · YALE UNIVERSITY · PI DERRE, ISABELLE · 2014 to 2018
$2.0M
NCI NIH HHS CA-0063244NIAID NIH HHS AI081694NIAID NIH HHS AI100759NIAID NIH HHS AI105561NIAID NIH HHS K08 AI091656NIAID NIH HHS K08AI091656NIAID NIH HHS K08 AI104766NIAID NIH HHS P01 AI090935NIAID NIH HHS P30 AI027763NIAID NIH HHS R01 AI039558NIAID NIH HHS R01 AI073770NIAID NIH HHS R01 AI081694NIAID NIH HHS R01 AI100759NIAID NIH HHS R01 AI101441NIAID NIH HHS R01AI101441NIAID NIH HHS R21 AI062768NIAID NIH HHS R21 AI105561NIAID NIH HHS T32 AI007334NIAID NIH HHS U19 AI106754NIAID NIH HHS U19 AI113187NIAID NIH HHS U54 AI081680NIAID NIH HHS U54AI081680NIGMS NIH HHS P50 GM081879NIGMS NIH HHS P50 GM082250PHS HHS A121844PHS HHS P0065678PHS HHS P01 090935PHS HHS P01 091575
6 · The paper itself

Abstract

Chlamydia trachomatis is a leading cause of genital and ocular infections for which no vaccine exists. Upon entry into host cells, C. trachomatis resides within a membrane-bound compartment—the inclusion—and secretes inclusion membrane proteins (Incs) that are thought to modulate the host-bacterium interface. To expand our understanding of Inc function(s), we subjected putative C. trachomatis Incs to affinity purification-mass spectroscopy (AP-MS). We identified Inc-human interactions for 38/58 Incs with enrichment in host processes consistent with Chlamydia's intracellular life cycle. There is significant overlap between Inc targets and viral proteins, suggesting common pathogenic mechanisms among obligate intracellular microbes. IncE binds to sorting nexins (SNXs) 5/6, components of the retromer, which relocalizes SNX5/6 to the inclusion membrane and augments inclusion membrane tubulation. Depletion of retromer components enhances progeny production, revealing that retromer restricts Chlamydia infection. This study demonstrates the value of proteomics in unveiling host-pathogen interactions in genetically challenging microbes.

Indexed as

Host-Pathogen InteractionsProtein Interaction MapsBacterial ProteinsChlamydia InfectionsChlamydia trachomatisHumansInclusion BodiesIntracellular MembranesProtein Interaction MappingProteomeBacterial ProteinsProteome

Identifiers

PMID26118995
PMCPMC4540348

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.