ArticleScientific reports2015
Fast, multi-frequency, and quantitative nanomechanical mapping of live cells using the atomic force microscope.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 116 citations in OpenAlex.
- Inline mechano-vibration holography for simultaneous phase and elasticity mapping of soft samples.Biomedical optics express · 2026Article
- Advances in nanomechanical property mapping by atomic force microscopy.Nanoscale advances · 2025Review
- Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker.ACS nano · 2025Article
- The Young's Modulus as a Mechanical Biomarker in AFM Experiments: A Tool for Cancer Diagnosis and Treatment Monitoring.Sensors (Basel, Switzerland) · 2025Review
- Noninvasive holographic sensor system for measuring stiffness of soft micro samples.Journal of biomedical optics · 2025Article
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- A Comprehensive Review on the Viscoelastic Parameters Used for Engineering Materials, Including Soft Materials, and the Relationships between Different Damping Parameters.Sensors (Basel, Switzerland) · 2024Review
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells.Current issues in molecular biology · 2024Article
- Light-induced modulation of viscoelastic properties in azobenzene polymers.Nanophotonics (Berlin, Germany) · 2024Article
- Atomic force microscopy-mediated mechanobiological profiling of complex human tissues.Biomaterials · 2023Review
- Cell wall dynamics: novel tools and research questions.Journal of experimental botany · 2023Review
- Ultra-wideband optical coherence elastography from acoustic to ultrasonic frequencies.Nature communications · 2023Article
- Cell wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties.Journal of experimental botany · 2023Article
- Uncovering the cytotoxic effects of air pollution with multi-modal imaging ofRoyal Society open science · 2023Review
- Determining Spatial Variability of Elastic Properties for Biological Samples Using AFM.Micromachines · 2023Review
- Article
- 3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers.Scientific reports · 2022Article
- Viscoelastic parameterization of human skin cells characterize material behavior at multiple timescales.Communications biology · 2022Article
- Frequency-dependent nanomechanical profiling for medical diagnosis.Beilstein journal of nanotechnology · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A longstanding goal in cellular mechanobiology has been to link dynamic biomolecular processes underpinning disease or morphogenesis to spatio-temporal changes in nanoscale mechanical properties such as viscoelasticity, surface tension, and adhesion. This requires the development of quantitative mechanical microscopy methods with high spatio-temporal resolution within a single cell. The Atomic Force Microscope (AFM) can map the heterogeneous mechanical properties of cells with high spatial resolution, however, the image acquisition time is 1-2 orders of magnitude longer than that required to study dynamic cellular processes. We present a technique that allows commercial AFM systems to map quantitatively the dynamically changing viscoelastic properties of live eukaryotic cells at widely separated frequencies over large areas (several 10's of microns) with spatial resolution equal to amplitude-modulation (AM-AFM) and with image acquisition times (tens of seconds) approaching those of speckle fluorescence methods. This represents a ~20 fold improvement in nanomechanical imaging throughput compared to AM-AFM and is fully compatible with emerging high speed AFM systems. This method is used to study the spatio-temporal mechanical response of MDA-MB-231 breast carcinoma cells to the inhibition of Syk protein tyrosine kinase giving insight into the signaling pathways by which Syk negatively regulates motility of highly invasive cancer cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.