ArticleApplied and environmental microbiology2015
Whole-Genome Sequencing Allows for Improved Identification of Persistent Listeria monocytogenes in Food-Associated Environments.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microbial Community Associations With Listeria monocytogenes in Food Processing Environments: A Systematic Review and Meta-Analysis.Comprehensive reviews in food science and food safety · 2025Pooled it
- Biofilm forming capacity, sanitizer tolerance, and genetic characterization of persistent and non-persistentBiofilm · 2026Article
- Retrospective Analysis of Historical Listeria monocytogenes Clinical Isolates, New York, USA, 2000-2021Emerging infectious diseases · 2025Review
- Predominance of L. monocytogenes Lineage I Clones in Wastewater, Ruminants, and Natural Environments.Environmental microbiology · 2025Article
- Application of Bioinformatics and Machine Learning Tools in Food Safety.Current nutrition reports · 2025Review
- Significance of Whole-Genome Sequencing for the Traceability of Foodborne Pathogens: During the Processing of Meat and Dairy Products.Foods (Basel, Switzerland) · 2025Review
- Population structure and gene flux ofMicrobial genomics · 2025Article
- Maternal-Fetal Listeriosis in China: Clinical and Genomic Characteristics From an ST8Infection and drug resistance · 2025Article
- Virulence Potential and Antimicrobial Resistance ofMicroorganisms · 2024Article
- Genomic characterization ofFrontiers in microbiology · 2024Article
- Review
- Assessment of the influence of selected stress factors on the growth and survival of Listeria monocytogenes.BMC microbiology · 2023Article
- Genetic diversity of Salmonella enterica isolated over 13 years from raw California almonds and from an almond orchard.PloS one · 2023Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Whole-Genome Sequencing-Based Characterization ofApplied and environmental microbiology · 2022Article
- Integrative Assessment of Reduced Listeria monocytogenes Susceptibility to Benzalkonium Chloride in Produce Processing Environments.Applied and environmental microbiology · 2022Article
- PervasiveApplied and environmental microbiology · 2022Review
- Article
- Phylogenetic and Phenotypic Analyses of a Collection of Food and Clinical Listeria monocytogenes Isolates Reveal Loss of Function of Sigma B from Several Clonal Complexes.Applied and environmental microbiology · 2022Article
- Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria.Biology · 2022Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the food-borne pathogen Listeria monocytogenes can persist in food associated environments, there are no whole-genome sequence (WGS) based methods to differentiate persistent from sporadic strains. Whole-genome sequencing of 188 isolates from a longitudinal study of L. monocytogenes in retail delis was used to (i) apply single-nucleotide polymorphism (SNP)-based phylogenetics for subtyping of L. monocytogenes, (ii) use SNP counts to differentiate persistent from repeatedly reintroduced strains, and (iii) identify genetic determinants of L. monocytogenes persistence. WGS analysis revealed three prophage regions that explained differences between three pairs of phylogenetically similar populations with pulsed-field gel electrophoresis types that differed by ≤3 bands. WGS-SNP-based phylogenetics found that putatively persistent L. monocytogenes represent SNP patterns (i) unique to a single retail deli, supporting persistence within the deli (11 clades), (ii) unique to a single state, supporting clonal spread within a state (7 clades), or (iii) spanning multiple states (5 clades). Isolates that formed one of 11 deli-specific clades differed by a median of 10 SNPs or fewer. Isolates from 12 putative persistence events had significantly fewer SNPs (median, 2 to 22 SNPs) than between isolates of the same subtype from other delis (median up to 77 SNPs), supporting persistence of the strain. In 13 events, nearly indistinguishable isolates (0 to 1 SNP) were found across multiple delis. No individual genes were enriched among persistent isolates compared to sporadic isolates. Our data show that WGS analysis improves food-borne pathogen subtyping and identification of persistent bacterial pathogens in food associated environments.
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