ArticleApplied and environmental microbiology2015
Use of the mCherry Fluorescent Protein To Study Intestinal Colonization by Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 in Mice.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Real-time tracking ofMicrobiology spectrum · 2026Article
- Genetic Toolbox Expansion Enables Constitutively Fluorescent Lacticaseibacillus rhamnosus for Functional Microbiome Research.Microbial biotechnology · 2026Article
- Antibacterial Activity of Zinc-Doped Hydroxyapatite and Vancomycin-Loaded Gelatin Nanoparticles against IntracellularACS applied nano materials · 2024Article
- Recent advances in genetic tools for engineering probiotic lactic acid bacteria.Bioscience reports · 2023Article
- Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces.Scientific reports · 2021Article
- Stable Recombinant-Gene Expression from aApplied and environmental microbiology · 2021Article
- Construction of an Integrated mCherry Red Fluorescent Protein Expression System for Labeling and Tracing inFrontiers in microbiology · 2021Article
- A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice byRSC advances · 2020Article
- Migration of Bacteriocins Across Gastrointestinal Epithelial and Vascular Endothelial Cells, as Determined Using In Vitro Simulations.Scientific reports · 2019Article
- Development of a novel selection/counter-selection system for chromosomal gene integrations and deletions in lactic acid bacteria.BMC molecular biology · 2019Article
- Stable genetic integration of a red fluorescent protein in a virulent Group AAccess microbiology · 2019Article
- Real-Time Detection of Riboflavin Production byFrontiers in microbiology · 2019Article
- In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.BMC microbiology · 2018Article
- Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species.Nano convergence · 2018Review
- Microencapsulation of Lactic Acid Bacteria Improves the Gastrointestinal Delivery andFrontiers in microbiology · 2018Article
- Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors.International journal of molecular sciences · 2017Article
- Optical Biosensing of Bacteria and Bacterial Communities.Journal of analysis and testing · 2017Article
- Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin.Microorganisms · 2016Review
- Fluorescent reporter systems for tracking probiotic lactic acid bacteria and bifidobacteria.World journal of microbiology & biotechnology · 2016Review
- Persistence and dynamics of fluorescentGut microbesArticle
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactic acid bacteria (LAB) are natural inhabitants of the gastrointestinal tract (GIT) of humans and animals, and some LAB species receive considerable attention due to their health benefits. Although many papers have been published on probiotic LAB, only a few reports have been published on the migration and colonization of the cells in the GIT. This is due mostly to the lack of efficient reporter systems. In this study, we report on the application of the fluorescent mCherry protein in the in vivo tagging of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423. The mCherry gene, encoding a red fluorescent protein (RFP), was integrated into a nonfunctional region on the genome of L. plantarum 423 by homologous recombination. In the case of E. mundtii ST4SA, the mCherry gene was cloned into the pGKV223D LAB/Escherichia coli expression vector. Expression of the mCherry gene did not alter the growth rate of the two strains and had no effect on bacteriocin production. Both strains colonized the cecum and colon of mice.
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