ArticleJournal of the American Society of Nephrology : JASN2016
Intravital Imaging Reveals Angiotensin II-Induced Transcytosis of Albumin by Podocytes.
Article in Journal of the American Society of Nephrology : JASN, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- GPR81 Regulates MCT1 Membrane Translocation Through a PKA-Dependent Signaling Pathway in Rat Podocytes.International journal of molecular sciences · 2026Article
- Urinary vesicle biomarkers and kidney function-results from the German AugUR study.Scientific reports · 2026Article
- Reactive oxygen species-evoked endoplasmic reticulum stress mediates albumin load-induced epithelial-mesenchymal transition in podocytes.Molecular biology reports · 2026Article
- Cardiovascular Autonomic Control in Normotensive Patients with Autosomal Dominant Polycystic Kidney Disease.Kidney360 · 2025Article
- Nephrotic Range Proteinuria as a Manifestation of Transplant Renal Artery Stenosis in Kidney Transplant Recipient.Kidney medicine · 2025Article
- The Life of a Kidney Podocyte.Acta physiologica (Oxford, England) · 2025Review
- Longitudinal intravital microscopy of the mouse kidney: inflammatory responses to abdominal imaging windows.American journal of physiology. Renal physiology · 2024Article
- The proteasome modulates endocytosis specifically in glomerular cells to promote kidney filtration.Nature communications · 2024Article
- Spatial composition and turnover of the main molecules in the adult glomerular basement membrane.Tissue barriers · 2023Review
- Ion channels and channelopathies in glomeruli.Physiological reviews · 2023Review
- Blocking CHOP-dependent TXNIP shuttling to mitochondria attenuates albuminuria and mitigates kidney injury in nephrotic syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Podocyte Endocytosis in Regulating the Glomerular Filtration Barrier.Frontiers in medicine · 2022Review
- Decreased Podocyte Vesicle Transcytosis and Albuminuria inInternational journal of molecular sciences · 2021Article
- Downregulation of megalin, cubilin, ClC-5 and podocin in Fabry nephropathy: potential implications in the decreased effectiveness of enzyme replacement therapy.Journal of nephrology · 2021Article
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Understanding the in vivo Fate of Advanced Materials by Imaging.Advanced functional materials · 2020Article
- Injured Podocytes Are Sensitized to Angiotensin II-Induced Calcium Signaling.Journal of the American Society of Nephrology : JASN · 2020Article
- Megalin: A bridge connecting kidney, the renin-angiotensin system, and atherosclerosis.Pharmacological research · 2020Review
- Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Overexpression of Myo1e promotes albumin endocytosis by mouse glomerular podocytes mediated by Dynamin.PeerJ · 2020Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Albuminuria is a hallmark of kidney disease of various etiologies and usually caused by deterioration of glomerular filtration barrier integrity. We recently showed that angiotensin II (Ang II) acutely increases albumin filtration in the healthy kidney. Here, we used intravital microscopy to assess the effects of Ang II on podocyte function in rats. Acute infusion of 30, 60, or 80 ng/kg per minute Ang II enhanced the endocytosis of albumin by activation of the type 1 Ang II receptor and resulted in an average (±SEM) of 3.7±2.2, 72.3±18.6 (P<0.001), and 239.4±34.6 µm(3) (P<0.001) albumin-containing vesicles per glomerulus, respectively, compared with none at baseline or 10 ng/kg per minute Ang II. Immunostaining of Ang II-infused kidneys confirmed the presence of albumin-containing vesicles, which colocalized with megalin, in podocin-positive cells. Furthermore, podocyte endocytosis of albumin was markedly reduced in the presence of gentamicin, a competitive inhibitor of megalin-dependent endocytosis. Ang II infusion increased the concentration of albumin in the subpodocyte space, a potential source for endocytic protein uptake, and gentamicin further increased this concentration. Some endocytic vesicles were acidified and colocalized with LysoTracker. Most vesicles migrated from the capillary to the apical aspect of the podocyte and were eventually released into the urinary space. This transcytosis accounted for approximately 10% of total albumin filtration. In summary, the transcellular transport of proteins across the podocyte constitutes a new pathway of glomerular protein filtration. Ang II enhances the endocytosis and transcytosis of plasma albumin by podocytes, which may eventually impair podocyte function.
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