ArticleEukaryotic cell2015
The Centriole Cartwheel Protein SAS-6 in Trypanosoma brucei Is Required for Probasal Body Biogenesis and Flagellum Assembly.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Axonemal dynein contributions to flagellar beat types and waveforms.bioRxiv : the preprint server for biology · 2025Article
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- Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency.Nature communications · 2025Article
- Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.Science (New York, N.Y.) · 2025Article
- NuSAP4 regulates chromosome segregation in Trypanosoma brucei by promoting bipolar spindle assembly.Communications biology · 2024Article
- Identification of 30 transition fibre proteins in Trypanosoma brucei reveals a complex and dynamic structure.Journal of cell science · 2024Article
- A kinesin-13 family kinesin in Trypanosoma brucei regulates cytokinesis and cytoskeleton morphogenesis by promoting microtubule bundling.PLoS pathogens · 2024Article
- Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.PLoS neglected tropical diseases · 2023Article
- Single p197 molecules of the mitochondrial genome segregation system ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defects.Nature communications · 2022Article
- The Trypanosomatids Cell Cycle: A Brief Report.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Cell Cycle, Telomeres, and Telomerase inCells · 2021Review
- Ultrastructure expansion microscopy inOpen biology · 2021Article
- Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome.PloS one · 2021Article
- Polo-like kinase in trypanosomes: an odd member out of the Polo family.Open biology · 2020Article
- Maintenance of hook complex integrity and centrin arm assembly facilitates flagellum inheritance inThe Journal of biological chemistry · 2020Article
- Basal Body Protein TbSAF1 Is Required for Microtubule Quartet Anchorage to the Basal Bodies inmBio · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
- BOH1 cooperates with Polo-like kinase to regulate flagellum inheritance and cytokinesis initiation inJournal of cell science · 2019Article
- Cellular landmarks of Trypanosoma brucei and Leishmania mexicana.Molecular and biochemical parasitology · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The centriole in eukaryotes functions as the cell's microtubule-organizing center (MTOC) to nucleate spindle assembly, and its biogenesis requires an evolutionarily conserved protein, SAS-6, which assembles the centriole cartwheel. Trypanosoma brucei, an early branching protozoan, possesses the basal body as its MTOC to nucleate flagellum biogenesis. However, little is known about the components of the basal body and their roles in basal body biogenesis and flagellum assembly. Here, we report that the T. brucei SAS-6 homolog, TbSAS-6, is localized to the mature basal body and the probasal body throughout the cell cycle. RNA interference (RNAi) of TbSAS-6 inhibited probasal body biogenesis, compromised flagellum assembly, and caused cytokinesis arrest. Surprisingly, overexpression of TbSAS-6 in T. brucei also impaired probasal body duplication and flagellum assembly, contrary to SAS-6 overexpression in humans, which produces supernumerary centrioles. Furthermore, we showed that depletion of T. brucei Polo-like kinase, TbPLK, or inhibition of TbPLK activity did not abolish TbSAS-6 localization to the basal body, in contrast to the essential role of Polo-like kinase in recruiting SAS-6 to centrioles in animals. Altogether, these results identified the essential role of TbSAS-6 in probasal body biogenesis and flagellum assembly and suggest the presence of a TbPLK-independent pathway governing basal body duplication in T. brucei.
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