ArticleProtoplasma2016
Evaluation of cytotoxicity, morphological alterations and oxidative stress in Chinook salmon cells exposed to copper oxide nanoparticles.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Oxidative Stress Biomarkers in the Shrimp Macrobrachium amazonicum (Heller, 1862): Assessment in an Environmental Preservation Area in the Brazilian Amazon.Environmental toxicology · 2026Article
- Comparative study of influence of Cu, CuO nanoparticles and CuScientific reports · 2024Article
- Is the trend toward a sustainable green synthesis of copper oxide nanoparticles completely safe for Oreochromis niloticus when compared to chemical ones?: using oxidative stress, bioaccumulation, and histological biomarkers.Environmental science and pollution research international · 2024Article
- Synthesis, Characterization, and Antimicrobial and Antiproliferative Effects of CuO-TiONanomaterials (Basel, Switzerland) · 2022Article
- Polyphenol effects on CuO-nanoparticle-mediated DNA damage, reactive oxygen species generation, and fibroblast cell death.Toxicology in vitro : an international journal published in association with BIBRA · 2022Article
- Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation.Frontiers in immunology · 2022Article
- The in vitro toxicity evaluation of halloysite nanotubes (HNTs) in human lung cells.Toxicological research · 2021Article
- Toxicity of Nanoparticles in Biomedical Application: Nanotoxicology.Journal of toxicology · 2021Review
- Copper(II) generates ROS and RNS, impairs antioxidant system and damages membrane and DNA in human blood cells.Environmental science and pollution research international · 2019Article
- Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila.Nanomaterials (Basel, Switzerland) · 2018Article
- Innate Immunity Provides Biomarkers of Health for Teleosts Exposed to Nanoparticles.Frontiers in immunology · 2018Review
- Oxidative Stress in the Muscles of the Fish Nile Tilapia Caused by Zinc Oxide Nanoparticles and Its Modulation by Vitamins C and E.Oxidative medicine and cellular longevity · 2018Article
- Highly efficient upconversion of ErScientific reports · 2017Article
- Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells.Environmental science and pollution research international · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current study is aimed to study cytotoxicity and oxidative stress mediated changes induced by copper oxide nanoparticles (CuO NPs) in Chinook salmon cells (CHSE-214). To this end, a number of biochemical responses are evaluated in CHSE-214 cells which are as follows [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] MTT, neutral red uptake (NRU), lactate dehydrogenase (LDH), protein carbonyl (PC), lipid peroxidation (LPO), oxidised glutathione (GSSG), reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione sulfo-transferase (GST), superoxide dismutase (SOD), catalase (CAT), 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and reactive oxygen species (ROS), respectively. The 50% inhibition concentration (IC50) of CuO NPs to CHSE-214 cells after 24 h exposure was found to be 19.026 μg ml(-1). Viability of cells was reduced by CuO NPs, and the decrease was dose dependent as revealed by the MTT and NRU assay. CHSE-214 cells exposed to CuO NPs induced morphological changes. Initially, cells started to detach from the surface (12 h), followed by polyhedric, fusiform appearance (19 h) and finally the cells started to shrink. Later, the cells started losing their cellular contents leading to their death only after 24 h. LDH, PC, LPO, GSH, GPx, GST, SOD, CAT, 8-OHdG and ROS responses were seen significantly increased with the increase in the concentration of CuO NPs when compared to their respective controls. However, significant decrease in GSSG was perceptible in CHSE-214 cells exposed to CuO NPs in a dose-dependent manner. Our data demonstrated that CuO NPs induced cytotoxicity in CHSE-214 cells through the mediation of oxidative stress. The current study provides a baseline for the CuO NPs-mediated cytotoxic assessment in CHSE-214 cells for the future studies.
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