Evidence map›Paper›PMID 26115456›Full record

ArticleSensors (Basel, Switzerland)2015

Non-Invasive Optical Sensor Based Approaches for Monitoring Virus Culture to Minimize BSL3 Laboratory Entry.

Viswanath Ragupathy, Mohan Kumar Hayuri Giri Setty, Yordan Kostov, Xudong Ge, Shaunak Uplekar, Indira Hewlett, Govind Rao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Viswanath RagupathyLMV/DETTD/OBRR/CBER/FDA, Silver Spring, MD 20993, USA. viswanath.ragupathy@fda.hhs.gov.
Mohan Kumar Hayuri Giri SettyLMV/DETTD/OBRR/CBER/FDA, Silver Spring, MD 20993, USA. Mohan.Haleyurgirisetty@fda.hhs.gov.
Yordan KostovCenter for Advanced Sensor Technology and Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, MD 21250, USA. kostov@umbc.edu.
Xudong GeCenter for Advanced Sensor Technology and Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, MD 21250, USA. xge1@umbc.edu.
Shaunak UplekarCenter for Advanced Sensor Technology and Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, MD 21250, USA. shaunak1@umbc.edu.
Indira HewlettLMV/DETTD/OBRR/CBER/FDA, Silver Spring, MD 20993, USA. Indira.Hewlett@fda.hhs.gov.
Govind RaoCenter for Advanced Sensor Technology and Department of Chemical, Biochemical and Environmental Engineering, University of Maryland, Baltimore County, MD 21250, USA. grao@umbc.edu.
University of Maryland, Baltimore County · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High titers of infectious viruses for vaccine and diagnostic reference panel development are made by infecting susceptible mammalian cells. Laboratory procedures are strictly performed in a Bio-Safety Level-3 (BSL3) laboratory and each entry and exit involves the use of  disposable Personnel Protective Equipment (PPE) to observe cell culture conditions. Routine PPE use involves significant recurring costs. Alternative non-invasive optical sensor based approaches to remotely monitor cell culture may provide a promising and cost effective approach to monitor infectious virus cultures resulting in lower disruption and costs. We report here the monitoring of high titer cultures of Human Immunodeficiency Virus-1 (HIV-1) and Herpes Simplex Virus-2 (HSV-2) remotely with the use of optical oxygen sensors aseptically placed inside the cell culture vessel. The replacement of culture media for cell and virus propagation and virus load monitoring was effectively performed using this fluorescent sensor and resulted in half the number of visits to the BSL3 lab (five versus ten).

Indexed as

Biomedical ResearchHerpesvirus 2, HumanHIV-1OxygenSpectrometry, FluorescenceVirus CultivationOxygenBSL3 usemonitoringoptical sensorvirus cell culture

Identifiers

PMID26115456
PMCPMC4541811
OpenAlexW1600460326

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.