Evidence map›Paper›PMID 26115014›Full record

ArticlePloS one2015

Pullulanase and Starch Synthase III Are Associated with Formation of Vitreous Endosperm in Quality Protein Maize.

Hao Wu, Kasi Clay, Stephanie S Thompson, Tracie A Hennen-Bierwagen, Bethany J Andrews, Bernd Zechmann, Bryan C Gibbon

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Crop resistant starch and genetic improvement: a review of recent advances.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018
    Review
  4. Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Hao WuDepartment of Biology, Baylor University, Waco, Texas, 76798, United States of America.
Kasi ClayDepartment of Biology, Baylor University, Waco, Texas, 76798, United States of America.
Stephanie S ThompsonDepartment of Biology, Baylor University, Waco, Texas, 76798, United States of America.
Tracie A Hennen-BierwagenIowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa, 50011, United States of America.
Bethany J AndrewsTexas A&M University, Department of Soil and Crop Sciences, College Station, Texas, 77843, United States of America.
Bernd ZechmannCenter for Microscopy and Imaging, Baylor University, Waco, Texas, 76798, United States of America.
Bryan C GibbonDepartment of Biological Sciences, Florida A&M University, Tallahassee, Florida, 32307, United States of America.
Baylor University · USFlorida Agricultural and Mechanical University · USIowa State University · USTexas A&M University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The opaque-2 (o2) mutation of maize increases lysine content, but the low seed density and soft texture of this type of mutant are undesirable. Lines with modifiers of the soft kernel phenotype (mo2) called "Quality Protein Maize" (QPM) have high lysine and kernel phenotypes similar to normal maize. Prior research indicated that the formation of vitreous endosperm in QPM might involve changes in starch granule structure. In this study, we focused on analysis of two starch biosynthetic enzymes that may influence kernel vitreousness. Analysis of recombinant inbred lines derived from a cross of W64Ao2 and K0326Y revealed that pullulanase activity had significant positive correlation with kernel vitreousness. We also found that decreased Starch Synthase III abundance may decrease the pullulanase activity and average glucan chain length given the same Zpu1 genotype. Therefore, Starch Synthase III could indirectly influence the kernel vitreousness by affecting pullulanase activity and coordinating with pullulanase to alter the glucan chain length distribution of amylopectin, resulting in different starch structural properties. The glucan chain length distribution had strong positive correlation with the polydispersity index of glucan chains, which was positively associated with the kernel vitreousness based on nonlinear regression analysis. Therefore, we propose that pullulanase and Starch Synthase III are two important factors responsible for the formation of the vitreous phenotype of QPM endosperms.

Indexed as

EndospermGlycoside HydrolasesPlant ProteinsStarch SynthaseZea maysGlycoside HydrolasesPlant ProteinspullulanaseStarch Synthase

Identifiers

PMID26115014
PMCPMC4482715
OpenAlexW817274907

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.