ArticleRNA biology2015
A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.
Article in RNA biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- A STING-based fluorescent polarization assay for monitoring activities of cyclic dinucleotide metabolizing enzymes.RSC chemical biology · 2021Article
- Article
- Tools and systems for evolutionary engineering of biomolecules and microorganisms.Journal of industrial microbiology & biotechnology · 2019Review
- Single-Cell Microscopy Reveals That Levels of Cyclic di-GMP Vary among Bacillus subtilis Subpopulations.Journal of bacteriology · 2019Article
- From fluorescent proteins to fluorogenic RNAs: Tools for imaging cellular macromolecules.Protein science : a publication of the Protein Society · 2019Review
- Identification of Small-Molecule Modulators of Diguanylate Cyclase by FRET-Based High-Throughput Screening.Chembiochem : a European journal of chemical biology · 2019Article
- Fluorescent Detection of the Ubiquitous Bacterial Messenger 3',5' Cyclic Diguanylic Acid by Using a Small Aromatic Molecule.Frontiers in microbiology · 2019Article
- Evaluating riboswitch optimality.Methods in enzymology · 2019Article
- Identification and characterization of nucleobase-modified aptamers by click-SELEX.Nature protocols · 2018Article
- Using Light-Activated Enzymes for Modulating Intracellular c-di-GMP Levels in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Opportunities in the design and application of RNA for gene expression control.Nucleic acids research · 2016Review
- Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophoretic mobility shift assay (EMSA) for c-di-GMP quantitation that required minimal engineering of the RNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.