ArticlePloS one2015
Pseudomonas aeruginosa Biofilm Response and Resistance to Cold Atmospheric Pressure Plasma Is Linked to the Redox-Active Molecule Phenazine.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
- Possible Applications of Cold Atmospheric Plasma in Otorhinolaryngology: A Narrative Review.Antioxidants (Basel, Switzerland) · 2026Review
- Antifungal Efficacy of Plasma-Activated Liquids againstACS omega · 2025Article
- The effects of ex vivo physical cold atmospheric plasma treatment on endothelial cells in human corneal explants and its potential for in vivo application in refractory corneal ulcers.Scientific reports · 2025Article
- Interactions between native soil microbiome and a synthetic microbial community reveals bacteria with persistent traits.mSystems · 2025Article
- Treatment of Clinically Important Bacteria With Cold Atmospheric Plasma.Microbial biotechnology · 2025Review
- Antimicrobial Activity of Cold Atmospheric Plasma on Bacterial Strains Derived from Patients with Diabetic Foot Ulcers.Journal of microbiology and biotechnology · 2024Article
- Bioaerosol Inactivation by a Cold Plasma Ionizer Coupled with an Electrostatic Precipitator.Microorganisms · 2024Article
- Synergistic effects of oxidative and acid stress on bacterial membranes of Escherichia coli and Staphylococcus simulans.Communications biology · 2024Article
- The effect of cold plasma on the treatment of external otitis: an experimental study in rats.AMB Express · 2024Article
- Guided Plasma Application in Dentistry-An Alternative to Antibiotic Therapy.Antibiotics (Basel, Switzerland) · 2024Article
- Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms.Scientific reports · 2024Article
- Cold atmospheric pressure plasma-antibiotic synergy inBiofilm · 2023Article
- Mechanisms of bacterial inhibition and tolerance around cold atmospheric plasma.Applied microbiology and biotechnology · 2023Review
- The Role of Cold Atmospheric Plasma in Wound Healing Processes in Critically Ill Patients.Journal of personalized medicine · 2023Review
- Pyocyanin biosynthesis protects Pseudomonas aeruginosa from nonthermal plasma inactivation.Microbial biotechnology · 2022Article
- Non-thermal plasma causesFrontiers in cellular and infection microbiology · 2022Article
- Article
- Interactions of plasma-activated water with biofilms: inactivation, dispersal effects and mechanisms of action.NPJ biofilms and microbiomes · 2021Review
- The Emerging Role of Cold Atmospheric Plasma in Implantology: A Review of the Literature.Nanomaterials (Basel, Switzerland) · 2020Review
- Nonthermal Plasma Induces the Viable-but-Nonculturable State in Staphylococcus aureus via Metabolic Suppression and the Oxidative Stress Response.Applied and environmental microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is an important opportunistic pathogen displaying high antibiotic resistance. Its resistance is in part due to its outstanding ability to form biofilms on a range of biotic and abiotic surfaces leading to difficult-to-treat, often long-term infections. Cold atmospheric plasma (CAP) is a new, promising antibacterial treatment to combat antibiotic-resistant bacteria. Plasma is ionized gas that has antibacterial properties through the generation of a mix of reactive oxygen and nitrogen species (RONS), excited molecules, charged particles and UV photons. Our results show the efficient removal of P. aeruginosa biofilms using a plasma jet (kINPen med), with no viable cells detected after 5 min treatment and no attached biofilm cells visible with confocal microscopy after 10 min plasma treatment. Because of its multi-factorial action, it is widely presumed that the development of bacterial resistance to plasma is unlikely. However, our results indicate that a short plasma treatment (3 min) may lead to the emergence of a small number of surviving cells exhibiting enhanced resistance to subsequent plasma exposure. Interestingly, these cells also exhibited a higher degree of resistance to hydrogen peroxide. Whole genome comparison between surviving cells and control cells revealed 10 distinct polymorphic regions, including four belonging to the redox active, antibiotic pigment phenazine. Subsequently, the interaction between phenazine production and CAP resistance was demonstrated in biofilms of transposon mutants disrupted in different phenazine pathway genes which exhibited significantly altered sensitivity to CAP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.