Evidence map›Paper›PMID 26114028›Full record

ArticleBiomedical optics express2015

Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration.

Vikas Trivedi, Thai V Truong, Le A Trinh, Daniel B Holland, Michael Liebling, Scott E Fraser

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
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  6. Review
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  10. Review
  11. Article
  12. Article
  13. Imaging the Cardiac Extracellular Matrix.Advances in experimental medicine and biology · 2018
    Review
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Vikas TrivediDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125, USA ; Translational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA ; Equal contribution.
Thai V TruongTranslational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA ; Equal contribution.
Le A TrinhTranslational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.
Daniel B HollandTranslational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.
Michael LieblingDepartment of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara CA 93111 USA ; Idiap Research Institute, 1920 Martigny, Switzerland.
Scott E FraserTranslational Imaging Center, University of Southern California, Molecular and Computational Biology, Los Angeles CA 90089, USA.

Funding

Functional analysis of neural crest & palate: Imaging craniofacial developmentU01DE020063 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FRASER, SCOTT E · 2009 to 2013
$2.9M
NIDCR NIH HHS U01 DE020063
6 · The paper itself

Abstract

We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.

Indexed as

(170.1420) Biology(170.3010) Image reconstruction techniques(180.0180) Microscopy(190.0190) Nonlinear optics

Identifiers

PMID26114028
PMCPMC4473743

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.